DESENVOLVIMENTO DA CRIOPRESERVAÇÃO DE EMBRIÕES EM CAPRINOS
Palavras-chave:
Capra hircus, Criobiologia, Reprodução.Resumo
A criopreservação de embriões permite a conservação do material genético de caprinos por períodos indefinidos de tempo. O método de congelação tradicional e a vitrificação são os principais métodos de criopreservação de embriões, diferindo pela velocidade de resfriamento e concentrações de crioprotetores. Além dos aspectos técnicos, o sistema de produção de embriões (in vitro, in vivo, micro-manipulação), a qualidade morfológica e o estádio de desenvolvimento são fatores determinantes para a eficiência da criopreservação. O objetivo desta revisão foi apresentar um breve histórico, aplicações, principais investigações e algumas perspectivas sobre a criopreservação de embriões em caprinos.Downloads
Referências
ABE, H., YAMASHITA, S., ITOH, T., SATOH, T., HOSHI, H. Ultrastructure of bovine embryos developed from in vitro-matured and –fertilized oocytes: comparative morphological evaluation of embryos cultured either in serum-free or in serum-supplemented medium. Mol. Reprod. Dev. v.53, p.325–335, 1999.
ABE, H., YAMASHITA, S., SATOH, T., HOSHI, H. Accumulation of cytoplasmic lipid droplets in bovine embryos and cryotolerance of embryos developed in different culture system using serum-free or serumcontaining media. Mol. Reprod. Dev. v.61, p.57–66, 2002.
AL YACOUB, A.N., GAULY, M., HOLTZ, W. Open pulled straw vitrification of goat embryos at various stages of development. Theriogenology, v.73, p.1018–1023, 2010.
ALI, J., SHELTON, J. N. Design of vitrification solutions for the cryopreservation of embryos. Journal of Reproduction and Fertility. v.99, p.471-477, 1993.
ANDRABI, S.M.H., MAXWELL, W.M.C. A review on reproductive biotechnologies for conservation of endangered mammalian species. Anim. Reprod. Sci. v.99, p.223–243, 2007.
ARAÚJO-LEMOS, P.F.B., FREITAS NETO, L.M., MELO, J.V., MOURA, M.T., LIMA, P.F., OLIVEIRA, M.A.L. Comparison of different cryoprotectant regimes forvitrification of ovine embryos produced in vivo. Small Ruminant Research. v.119, p.100-106, 2014.
ARAÚJO-LEMOS, P.F.B, FREITAS NETO, L.M., MOURA, M.T., MELO, J.V., LIMA, P.F., OLIVEIRA, M.A.L. Comparison of vitrification and conventional freezing for cryopreservation of caprine embryos. Zygote. p.1–9, 2015.
BAKHTARI, A., RAHMANI, H., BONAKDAR, E., JAFARPOUR, F., ASGARI, V., HOSSEINI, S., HAJIAN, M., EDRISS, M., NASR-ESFAHAN, M. The interfering effects of superovulation and vitrification upon some important epigenetic biomarkers in mouse blastocyst. Cryobiology. v.69, p.419–427, 2014.
BEGIN, I., BHATIA, B., BALDASSARE, H., DINNYES, A., KEEFER, C.L. Cryopreservation of goat oocytes and in vivo derived 2-to 4-cell embryos using the cryoloop (CLV) and solid-surface vitrification (SSV) methods. Theriogenology v.59, p.1839-1850, 2003.
BETTERIDGE, K.J. Farm animal embryo technologies: Achievements and perspectives. Theriogenology. v.65, p.905–913, 2006.
BEZERRA, F.S., CASTELO, T.S., ALVES, H.M., OLIVEIRA, I.R., LIMA, G.L., PEIXOTO, G.C., BEZERRA, A.C., SILVA, A.R. Objective assessment of the cryoprotective effects of dimethylformamide for freezing goat semen. Cryobiology. v.63, p.263-266, 2011.
BILTON, R.J., MOORE, N.W.In vitro culture, storage and transfer of goat embryos. Aust J Biol Sci. v.29, p.125-9, 1976.
BLOCK, J., BONILLA, L., HANSEN, P.J. Effect of addition of hyaluronan to embryo culture medium on survival of bovine embryos in vitro following vitrification and establishment of pregnancy after transfer to recipients. Theriogenology. v.7, p.1063–1071, 2009.
BOONKUSOL, D., GAL, A.B., BODO, S., GORHONY, B., KITIYANANT, Y., DINNYES, A.Gene expression profiles and in vitro development following vitrification of pronuclear and 8-cell stage mouse embryos. Mol Reprod Dev. v.73, p.700-708, 2006.
CHEMINEAU, P., PROCUREUR, R., COGNIÉ, Y., LEFÈVRE, P.C., LOCATELLI, A., CHUPIN, D. Production, freezing, and transfer of embryos from a bluetongue-infected goat herd without bluetongue transmission. Theriogenology. v.26, p.279-290, 1986.
CHJAN, R.C.; KUWAYAMA, M.; TAN, L.; TAN, J.; KATO, O.; NAGAS, T. High survival rate of bovine oocytes matured in vitro following vitrification. J. Reprod. Dev. v.6, p.685-696, 2004.
COGNIÉ, Y., BARIL, G., POULIN, N., MERMILLOD, P. Current status of embryos technologies in sheep and goat. Theriogenology. v.59, p.171-188, 2003.
DATTENA, M., MARA, L., BIN, T.A.A., CAPPAI, P. Lambing rate using vitrified blastocysts is improved by culture with BSA and hyaluronan. Mol. Reprod. Dev. v.1, p.42–47, 2007.
DHALI, A., ANCHAMPARUTHY, V.M., BUTLER, S.P., PEARSON, R.E., MULLARKY, I.K., GWAZDAUSKAS, F.C. Theriogenol=ogy. v.68, p.1292-1298, 2007.
DIEZ, C., LE BOURHIS, Y., HEYMAN, Y., RENARD, J.P. Effect of partial lipid removal from in vitro produced bovine zygotes on further development in vitro and on the freezing tolerance of blastocysts. Theriogenology. v.45, p.166, 1996.
DOBRINSKY, J.R. Advancements in cryopreservation of domestic animal embryos. Theriogenology. v.57, p.285-302, 2002.
EL-GAYAR M., HOLTZ W. Technical note: Vitrification of goat embryos by the open pulled-straw method. J Anim Sci. v.79, p.2436-2438, 2001.
FIÉNI F., BECKERS, J.P., BUGGIN, M., BRUYAS, J.F., PERRIN, J., DAUBIÉ, M., TAINTURIER, D. Evaluation of cryopreservation techniques for goat embryos. Reprod. Nutr. Dev. v.35, p.367-373, 1995.
FOUNTAIN, D.M., RALSTON, M., HIGGINS, N., GORLIN, J.B., UHL L., WHEELER C., ANTIN J.H., CHURCHILL W.H., BENJAMIN R.J. Liquid nitrogen freezers: a potential source of microbial contamination of hematopoietic stem cell components. Transfusion. v.37, p.585-591, 1997.
GAMA, L.T., BRESSANA, M.C. Biotechnology applications for the sustainable management of goat genetic resources. Small Ruminant Research. v.98, p.133–146, 2011.
GARCIA-GARCIA, R.M., GONZALEZ-BULNES, A., DOMINGUEZ, V., VEIGALOPEZ, A., COCERO, M.J. Survival of frozen-thawed sheep embryos cryopreserved at cleavage stages. Cryobiology. v.52, p.108-113, 2006.
GIBBONS, A., CUETO, M.I., BONNET, F.P. A simple vitrification technique for sheep and goat embryo cryopreservation. Small Ruminant Research. v.95, p.61–64, 2011.
GUIGNOT, F., BOUTTIER, A., BARIL, G., SALVETTI, P., PIGNON, P., BECKERS, J.F., TOUZÉ, J.L., COGNIÉ, J., TRALDI, A.S., COGNIÉ, Y., MERMILLOD, P. Improved vitrification method allowing direct transfer of goat embryos. Theriogenology. v.66, p.1004-1011, 2006.
GUIGNOT, F., PERREAU, C., CAVARROC, C., TOUZÉ, J.L., POUGNARD, J.L., DUPONT, F., BECKERS, J.F., RÉMY, B., BABILLIOT, J.M., BED’HOM, B., LAMORINIÉRE, J.M., MERMILLOD, P., BARIL, G. Sex and PRNP Genotype Determination in Preimplantation Caprine Embryos. Reprod Dom Anim. v.46, p.656–663, 2011.
HOLTZ, W. Recent developments in assisted reproduction in goats. Small Ruminant Research. v.60, p.95-110, 2005.
HONG, Q.H., TIAN, S.J., ZHU, S.E., FENG, J.Z., YAN, C.L., ZHAO, X.M., LIU, G.S., ZHENG, S.M. Vitrification of Boer Goat Morulae and Early Blastocysts by Straw and Open-Pulled Straw Method. Reprod Dom Anim v.42, p.34–38, 2007.
KASAI, M., MUKAIDA, T. Cryopreservation of animal and human embryos by vitrification. Reproductive Bio Medicine Online. v.9, p.164-170, 2004.
KULESHOVA, L.L., MACFARLANE, D.R., TROUNSON, A.O., SHAW, J.M. Sugars exert a major influence on vitrification properties of ethylene glycol-based solutions and have toxicity to embryos and oocytes. Cryobiology v.38, p.119-130, 1999.
LE GALL, F., BARIL, G., VALLET, J.C., LEBOEUF, B. In vivo and in vitro survival of goat embryos after freezing with ethylene glycol or glycerol. Theriogenology. v.40, p.771-777, 1993.
LEIBO, S.P., POLLARD, J.W., MARTINO, A. Chilling and freezing sensitivity of reassembled in vitro derived bovine embryos. Theriogenology. v.43, p.265, 1995.
LEIBO, S.P., MARTINO, A., KOBAYASHI, S., POLLARD, J.W. Stage dependent sensitivity of oocytes and embryos to low temperatures. Anim. Reprod. Sci. v.42, p.45-53, 1996.
LEIBO, S.P. Cryopreservation of oocytes and embryos: Optimization by theoretical versus empirical analysis. Theriogenology. v.69, p.37–47, 2008.
LEONI, G.G., SUCCU, S., SATTA, V., PAOLO, M., BOGLIOLO, L., BEBBERE, D., SPEZZIGU, A., MADEDDU, M., BERLINGUER, F., LEDDA, S., NAITANA, S.In vitro production and cryotolerance of prepubertal and adult goat blastocysts obtained from oocytes collected by laparoscopic oocyte-pick-up (LOPU) after FSH treatment. Reprod Fertil Dev. v.21, p.901-908, 2009.
LI, R., CAMERON, W.N., BATT, P.A., TROUNSON, A.O. Maximal survival of frozen goat embryos is attained at the expanded, hatching and hatched blastocyst stages of development. Reprod. Fertil. Dev. v.2, p.345-350, 1990.
LOUTRADI, K.E., KOLIBIANAKIS, E.M., VENETIS, C.A., PAPANIKOLAOU, E.G., PADOS, G., BONTIS, I., TARLATZIS, B.C. Cryopreservation of human embryos by vitrification or slow freezing: a systematic review and meta-analysis. Fertil Steril. v.90, p.186-193, 2008.
MARA L., SARA CASU, CARTA A., DATTENA M. Cryobanking of farm animal gametes and embryos as a means of conserving livestock genetics. Animal Reproduction Science. v.138, p.25–38, 2013.
MARTEMUCCI, G., D’ALESSANDRO, A.G. Efficiency of FSH/LH treatments for in vivo production of embryos and their cryopreservation by different methods in goats. Small Ruminant Research. v.114, p.264–271, 2013.
MARTINO, A., SONGSASEN, N., LEIBO, S.P. Development into blastocysts of bovine oocytes cryopreserved by ultra-rapid cooling. Biol. Reprod. v.54, p.1059–1069, 1996.
MARTINO, N.A., DELL’AQUILA, M.E., CARDONE, R.A., SOMOSKOI, B., LACALANDRA, G.M., CSEH, S. Vitrification preserves chromatin integrity, bioenergy potential and oxidative parameters in mouse embryos. Reprod Biol Endocrinol. v.11, p.27, 2013.
MASSIP, A. Cryopreservation of embryos of farm animals. Reprod. Dom. Anim. v.36, p.49-55, 2001.
MORATÓ, R., ROMAGUERA, R., IZQUIERDO, D., PARAMIO, M.T., MOGAS, T.,F. Vitrification of in vitro produced goat blastocysts: Effects of oocyte donor age and development stage. Cryobiology. v.63, p.240–244, 2011.
NOWSHARI, M.A., HOLTZ, W. Transfer of Split Goat Embryos Without Zonae Pellucidae Either Fresh or After Freezing. Journal Animal Science. v.71, p.3403-3408, 1993.
NOWSHARI, M.A., HOLTZ, W. In vitro culture of goat morulae to blastocysts before freezing. Theriogenology. v.44, p.983-988, 1995.
PARAMIO, M., IZQUIERDO, D. Assisted reproduction technologies in goats. Small Ruminant Research. v.121, p.21–26, 2014.
POLGE, C.; SMITH, A.U.; PARKES, A.S. Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature, v.164, p.666-666, 1949.
PULS-KLEINGELD, M., NOWSHARI, M.A., HOLTZ, W. Cryopreservation of goat embryos by the one- step or three-step equilibration procedure. In: Lokeshwar RR (ed), Recent Advances in Goat Production. New-Delhi: Nutan Printers, 1992. pp.1388-1391.
RALL, W.F., FAHY, G.M.Ice-free cryopreservation of mouse embryos at -196 degrees C by vitrification. Nature, v.313, p.573-575, 1985.
RAO, V.H., SARMAH, B.C., AGRAWAL, K.P., ANSARI, M.R., BHATTACHARYYA, N.K. Survival of goat embryos frozen and thawed rapidly. Animal Reproduction Science, v.16, p.261-264, 1988.
RODRÍGUEZ-DORTA, N., COGNIÉ, Y., GONZÁLEZ, F., POULIN, N., GUIGNOT, F., TOUZÉ, J.L., BARIL, G., CABRERA, F., ALAMO, D., BATISTA, M., GRACIA, A., MERMILLOD, P. Effect of coculture with oviduct epithelial cells on viability after transfer of vitrified in vitro produced goat embryos. Theriogenology. v.68, p.908-913, 2007.
SAENZ-DE-JUANO, M.D., MARCO-JIMENEZ, F., SCHMALTZ-PANNEAU, B., JIMENEZ-TRIGOS, E., VIUDES-DE-CASTRO, M.P., PEÑARANDA, D.S., JOUNEAU, L., LECARDONNEL, J., LAVARA, R., NATURIL-ALFONSO, C., DURANTHON, V., VICENTE, J.S. Vitrification alters rabbit foetal placenta at transcriptomic and proteomic level. Reproduction, v.147, p.789-801, 2014.
SEIDEL, G.E. Jr. Modifying oocytes and embryos to improve their cryopreservation. Theriogenology v.65, p.228–235, 2006.
SOUZA-FABJAN, J.M.G., PANNEAU, B., DUFFARD, N., LOCATELLI, Y., FIGUEIREDO, J.R., FREITAS, V.J.F., MERMILLOD, P. In vitro production of small ruminant embryos: Late improvements and further research. Theriogenology. v.81, p.1149–1162, 2014.
TIBARY, A., ANOUASSI, A., KHATIR, H. Update on reproductive biotechnologies in small ruminants and camelids. Theriogenology. v.64, p.618–638, 2005.
TRALDI, A.S., LEBOEUF, B., COGNIÈ, Y., POULIN, N., MERMILLOD, P. Comparative results of in vitro and in vivo survival of vitrified in vitro produced goat and sheep embryos. Theriogenology. p.175, 1999.
TSUNODA, Y., TOKUNAGA, T., OKUBO, Y., SUGIE, T. Beneficial effect of agar for the frozen storage of bisected embryos. Theriogenology, v.28, p.317-322, 1987.
VAJTA G., HOLM P., KUWAYAMA M., BOOTH P.J., JACOBSEN H., GREVE T., CALLESEN H. Open pulled straw (ops) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol Reprod Dev. v.1, p.53-58, 1998.
VAJTA, G. Vitrification of oocytes and embryos of domestic animals. Anim. Reprod. Sci. v.60-61, p.357-364, 2000.
VAJTA, G., KUWAYAMA, M. Improving cryopreservation systems. Theriogenology. v.65, p.236-244, 2006.
WHITTINGHAM, D.G., ALIBI, S.P., MAZUR, P. Survival of mouse embryos frozen to -196°C and -269°C. Science. v.178, p.411-414, 1972.
YAVIN, S., ARAV, A. Measurement of essential physical properties of vitrification solutions. Theriogenology. v.67, p.81-89, 2007.
YUSWIATI, E., HOLTZ, W. Successful transfer of vitrified goat embryos. Theriogenology. v.34, p.629-632, 1990.
WILMUT, I.The effect of cooling rate, warming rate, cryoprotective agent and stage of development on survival of mouse embryos during freezing and thawing. Life Sci II. v.11, p.1071-1079, 1972a.
WILMUT, I.The low temperature preservation of mammalian embryos. J Reprod Fertil. v.31, p.513-514, 1972b.
WILMUT, I., ROWSON, L.E. Experiments on the low-temperature preservation of cow embryos. Vet Rec. v.92, p.686-690, 1973.
