THE EVOLVING PICTURE IN OBTAINING GENETICALLY MODIFIED LIVESTOCK
Palavras-chave:
Animais de produção, OGM, Transformação, Transgênese, DNA recombinante.Resumo
Animais de produção geneticamente modificados são aqueles que tiveram uma sequência de DNA endógena modificada ou um DNA exógeno introduzido em seu genoma. Animais de produção geneticamente modificados apresentam grande potencial como modelo para estudo de doenças humanas, para produção mais eficiente de carne e derivados do leite, para xenotransplante e para produção de produtos sob grande demanda para saúde humana. Diferentes abordagens têm sido descritas para obtenção de animais de produção geneticamente modificados, as quais apresentam eficiências, vantagens e limitações variáveis. O objetivo da revisão é descrever o histórico da obtenção de animais de produção geneticamente modificados, os principais obstáculos, abordagens atuais e perspectiva futuras sobre a tecnologia.Downloads
Referências
ANZAR, M.; BUHR, M.M. Spontaneous uptake of exogenous DNA by bull spermatozoa. Theriogenology, 65: 683-690, 2006.
BAGUISI, A.; BEHBOODI, E.; MELICAN, D.T.; POLLOCK, J.S.; DESTREMPES, M.M.; CAMMUSO, C.; WILLIAMS, J.L.; NIMS, S.D.; PORTER, C.A.; MIDURA, P.; PALACIOS, M.J.; AYRES, S.L.; DENNISTON, R.S.; HAYES, M.L.; ZIOMEK, C.A.; MEADE, H.M.; GODKE, R.A.; GAVIN, W.G.; OVERSTRÖM, E.W.; ECHELARD, Y. Production of goats
by somatic cell nuclear transfer. Nat Biotechnol, 17: 456-461, 1999.
BALDASSARRE, H.; WANG, B.; KAFIDI, N.; GAUTHIER, M.; NEVEU, N.; LAPOINTE, J.; SNEEK, L.; LEDUC, M.; DUGUAY, F.; ZHOU, J.F.; LAZARIS, A.; KARATZAS, C.N. Production of transgenic goats by pronuclear microinjection of in vitro produced zygotes derived from oocytes recovered by laparoscopy. Theriogenology, 59: 831-839, 2003.
BALDASSARRE, H.; WANG, B.; PIERSON, J.; NEVEU, N.; SNEEK, L.; LAPOINTE, J.; COTE, F.; KAFIDI, N.; KEEFER, C.L.; LAZARIS, A.; KARATZAS, C.N. Prepubertal propagation of transgenic cloned goats by laparoscopic ovum pick-up and in vitro embryo production. Cloning Stem Cells, 6: 25-29, 2004.
BEHBOODI, E.; LAM, L.; GAVIN, W.G.; BONDAREVA, A.; DOBRINSKI, I. Goat embryonic stem-like cell derivation and characterization. Methods Mol Biol., 1074: 51-67, 2013.
BEVACQUA, R.J.; FERNANDEZ-MARTÍN, R.; SAVY, V.; CANEL, N.G.; GISMONDI, M.I.; KUES, W.A.; CARLSON, D.F.; FAHRENKRUG, S.C.; NIEMANN, H.; TABOGA, O.A.; FERRARIS, S.; SALAMONE, D.F. Efficient edition of the bovine PRNP prion gene in somatic cells and IVF embryos using the CRISPR/Cas9 system. Theriogenology, 86: 1886-1896, 2016.
BLECK, G.T.; WHITE, B.R.; MILLER, D.J.; WHEELER, M.B. Production of bovine alpha- lactalbumin in the milk of transgenic pigs. J Anim Sci., 76: 3072-3078, 1998.
BONDIOLI, K.; RAMSOONDAR, J.; WILLIAMS, B.; COSTA, C.; FODOR, W. Cloned pigs generated from cultured skin fibroblasts derived from a H-transferase transgenic boar. Mol Reprod Dev., 60: 189-195, 2001.
BORDIGNON, V.; KEYSTON, R.; LAZARIS, A.; BILODEAU, A.S.; PONTES, J.H.; ARNOLD, D.; FECTEAU, G.; KEEFER, C.; SMITH, L.C. Transgene expression of green fluorescent protein and germ line transmission in cloned calves derived from in vitro- transfected somatic cells. Biol. Reprod., 68: 2013-2023, 2003.
BRACKETT, B.G.; BARANSKA, W.; SAWICKI, W.; KOPROWSKI, H. Uptake of heterologous genome by mammalian spermatozoa and its transfer to ova through fertilization. PNAS, 68: 353-357, 1971.
BRADLEY, A.; EVANS, M.; KAUFMAN, M.H.; ROBERTSON, E. Formation of germ–line chimaeras from embryo–derived teratocarcinoma cell lines. Nature, 309: 255–256,1984.
BRINSTER, R.L.; CHEN, H.Y.; TRUMBAUER, M.E.; YAGLE, M.K.; PALMITER, R.D. Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. PNAS, 82: 4438-4442, 1985.
BROPHY, B.; SMOLENSKI, G.; WHEELER, T.; WELLS, D.; L’HUILLIER, P.; LAIBLE, G. Cloned transgenic cattle produce milk with higher levels of beta-casein and kappa-casein. Nat Biotechnol, 21: 157-162, 2003.
BRUFORD, M.W.; BRADLEY, D.G.; LUIKART, G. DNA markers reveal the complexity of livestock domestication. Nat Rev Genet, 4: 900-910, 2003.
BUEHR, M.; MEEK, S.; BLAIR, K.; YANG, J.; URE, J.; SILVA, J.; MCLAY, R.; HALL, J.; YING, Q.L.; SMITH, A. Capture of authentic embryonic stem cells from rat blastocysts. Cell, 135: 1287-1298, 2008.
CAPECCHI, M.R. The new mouse genetics: altering the genome by gene targeting. Trend Genet, 5: 70-76, 1989.
CAPECCHI, M.R. Gene targeting in mice: functional analysis of the mammalian genome for the twenty-first century. Nat Rev Genet, 6: 507-512, 2005.
CHAN, A.W.; HOMAN, E.J.; BALLOU, L.U.; BURNS, J.C.; BREMEL, R.D. Transgenic cattle produced by reverse-transcribed gene transfer in oocytes. PNAS, 95: 14028-14033, 1998.
CHEN, F.; WANG, Y.; YUAN, Y.; ZHANG, W.; REN, Z.; JIN, Y.; LIU, X.; XIONG, Q.; CHEN, Q.; ZHANG, M.; LI, X.; ZHAO, L.; LI, Z.; WU, Z.; ZHANG, Y.; HU, F.; HUANG, J.; LI, R.; DAI, Y. Generation of B cell-deficient pigs by highly efficient CRISPR/Cas9-mediated gene targeting. J Genet Genomics, 42: 437-444, 2015A.
CHEN, X.; YE, S.; YING, Q.L. Stem cell maintenance by manipulating signaling pathways: past, current and future. BMB Rep, 48: 668-676, 2015B.
CIBELLI, J.B.; STICE, S.L.; GOLUEKE, P.J.; KANE, J.J.; JERRY, J.; BLACKWELL, C.; PONCE DE LEóN, F.A.; ROBL, J.M. Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Science, 280: 1256-1258, 1998.
CIBELLI, J.B.; STICE, S.L.; GOLUEKE, P.J.; KANE, J.J.; JERRY, J.; BLACKWELL, C.; PONCE DE LEóN, F.A.; ROBL, J.M. Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells. Nat Biotech, 16: 642-646, 1998.
CLARK, A.J. Generation of transgenic livestock by pronuclear injection. Methods Mol Biol, 180: 273-287, 2002.
COUSENS, C.; CARVER, A.S.; WILMUT, I.; COLMAN, A.; GARNER, I.; O’NEILL, G.T. Use of PCR-based methods for selection of integrated transgenes in preimplantation embryos. Mol Reprod Dev, 39: 384-391, 1994.
DAI, Y.; VAUGHT, T.D.; BOONE, J.; CHEN, S.H.; PHELPS, C.J.; BALL, S.; MONAHAN, J.A.; JOBST, P.M.; MCCREATH, K.J.; LAMBORN, A.E.; COWELL-LUCERO, J.L.; WELLS, K.D.; COLMAN, A.; POLEJAEVA, I.A.; AYARES, D.L. Targeted disruption of the alpha1,3-galactosyltransferase gene in cloned pigs. Nat Biotechnol, 20: 251-255, 2002.
DENNING, C.; DICKINSON, P.; BURL, S.; WYLIE, D.; FLETCHER, J.; CLARK, A.J. Gene targeting in primary fetal fibroblasts from sheep and pig. Cloning and Stem Cells, 3: 221-231, 2001.
EGGAN, K.; RODE, A.; JENTSCH, I.; SAMUEL, C.; HENNEK, T.; TINTRUP, H.; ZEVNIK, B.; ERWIN, J.; LORING, J.; JACKSON-GRUSBY, L.; SPEICHER, M.R.;KUEHN, R.; JAENISCH, R. Male and female mice derived from the same embryonic stem cell clone by tetraploid embryo complementation. Nat Biotechnol, 20: 455-459, 2002.
EGHBALSAIED, S.; GHAEDI, K.; LAIBLE, G.; HOSSEINI, S.M.; FOROUZANFAR, M.; HAJIAN, M.; OBACK, F.; NASR-ESFAHANI, M.H.; OBACK, B. Exposure to DNA is insufficient for in vitro transgenesis of live bovine sperm and embryos. Reproduction, 145: 97-108, 2003.
EVANS, M.J.; KAUFMAN, M.H. Establishment in culture of pluripotential cells from mouse embryos. Nature, 292: 154–156, 1981.
EYESTONE, W.H. Challenges and progress in the production of transgenic cattle. Reprod Fert Dev, 6: 647-652, 1994.
EYESTONE, W.H. Production and breeding of transgenic cattle using in vitro embryo production technology. Theriogenology, 51: 509-517, 1999.
EZASHI, T.; YUAN, Y.; ROBERTS, R.M. Pluripotent Stem Cells from Domesticated Mammals. Ann Rev Anim Biosciences, 4: 223-253, 2016.
FENG, W.; DAI, Y.; MOU, L.; COOPER, D.K.; SHI, D.; CAI, Z. The potential of the combination of CRISPR/Cas9 and pluripotent stem cells to provide human organs from chimaeric pigs. Int J Mol Sci, 16: 6545-6556, 2015.
FLISIKOWSKA, T.; KIND, A.; SCHNIEKE, A. Pigs as models of human cancers. Theriogenology, 86: 433-437, 2016.
FREITAS, V.J.F.; SEROVA, I.A.; MOURA, R.R.; ANDREEVA, L.E.; MELO, L.M.; TEIXEIRA, D.I.A.; PEREIRA, A.F.; LOPES-JR, E.S.; DIAS, L.P.B.; NUNES-PINHEIRO, D.C.S.; SOUSA, F.C.; ALCâNTARA-NETO, A.S.; ALBUQUERQUE, E.S.; MELO, C.H.S.; RODRIGUES, V.H.V.; BATISTA, R.I.T.P.; DVORYANCHIKOV, G.A.; SEROV, O.L. The establishment of two transgenic goat lines for mammary gland hG-CSF expression. Small Ruminant Research, 105: 105-113, 2012.
GANDOLFI, F. Sperm-mediated transgenesis. Theriogenology, 53: 127-137, 2000.
GARCÍA-ROSELLÓ, E.; GARCÍA-MENGUAL, E.; COY, P.; ALFONSO, J.; SILVESTRE, M.A. Intracytoplasmic sperm injection in livestock species: an update. Reprod Domest Anim, 44: 143-151, 2009.
GIANOLA, D.; ROSA, G.J. One hundred years of statistical developments in animal breeding. Annu Rev Anim Biosci., 3: 19-56, 2015.
GIL, M.A.; CUELLO, C.; PARRILLA, I.; VAZQUEZ, J.M.; ROCA, J.; MARTINEZ, E.A. Advances in swine in vitro embryo production technologies. Reprod Domest Anim, 45 (Supplement 2): 40-48, 2010.
GORDON, J.W.; SCANGOS, G.A.; PLOTKIN, D.J.; BARBOSA, J.A.; RUDDLE, F.H. Genetic transformation of mouse embryos by microinjection of purified DNA. PNAS, 77: 7380-7384, 1980.
GOSSLER, A.; DOETSCHMAN, T.; KORN, R.; SERFLING, E.; KEMLER, R. Transgenesis by means of blastocyst-derived embryonic stem cell lines. PNAS, 83: 9065-9069, 1986.
HABERMANN, F.A.; WUENSCH, A.; SINOWATZ, F.; WOLF, E. Reporter genes for embryogenesis research in livestock species. Theriogenology, 68: S116-124, 2007.
HAMMER, R.E.; PURSEL, V.G.; REXROAD, C.E. JR; WALL, R.J.; BOLT, D.J.; EBERT, K.M.; PALMITER, R.D.; BRINSTER, R.L. Production of transgenic rabbits, sheep and pigs by microinjection. Nature, 315: 680-683, 1985.
HASKELL, R.E.; BOWEN, R.A. Efficient production of transgenic cattle by retroviral infection of early embryos. Mol Reprod Dev, 40: 386-390, 1995.
HAYES, B.J.; LEWIN, H.A.; GODDARD, M.E. The future of livestock breeding: genomic selection for efficiency, reduced emissions intensity, and adaptation. Trends Genet, 29: 206- 214, 2013.
HE, W.; KONG, Q.; SHI, Y.; XIE, B.; JIAO, M.; HUANG, T.; GUO, S.; HU, K.; LIU, Z.Generation and developmental characteristics of porcine tetraploid embryos and tetraploid/ diploid chimeric embryos. Genomics Proteomics Bioinformatics, 11: 327-333, 2013.
HENDERSON, C.R. Best linear unbiased estimation and prediction under a selection model. Biometrics, 31: 423-447, 1975.
HOCHEDLINGER, K.; JAENISCH, R. Nuclear transplantation: lessons from frogs and mice. Curr Opin Cell Biol, 14: 741-748, 2002.
HOFMANN, A.; KESSLER, B.; EWERLING, S.; WEPPERT, M.; VOGG, B.; LUDWIG, H.; STOJKOVIC, M.; BOELHAUVE, M.; BREM, G.; WOLF, E.; PFEIFER, A. Efficient transgenesis in farm animals by lentiviral vectors. EMBO Reports, 4: 1054-1060, 2003.
HOFMANN, A.; ZAKHARTCHENKO, V.; WEPPERT, M.; SEBALD, H.;
WENIGERKIND, H.; BREM, G.; WOLF, E.; PFEIFER, A. Generation of transgenic cattle by lentiviral gene transfer into oocytes. Biol Reprod, 71: 405-409, 2004.
JAENISCH, R.; MINTZ, B. Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA. PNAS, 71: 1250-1254, 1974.
JäHNER, D.; STUHLMANN, H.; STEWART, C.L.; HARBERS, K.; LÖHLER, J.; SIMON, I.; JAENISCH, R. De novo methylation and expression of retroviral genomes during mouse embryogenesis. Nature, 298: 623-628, 1982.
JULLIEN, J.; PASQUE, V.; HALLEY-STOTT, R.P.; MIYAMOTO, K.; GURDON, J.B. Mechanisms of nuclear reprogramming by eggs and oocytes: a deterministic process? Nat Rev Mol Cell Biol, 12: 453-459, 2011.
KEEFER, C.L. Artificial cloning of domestic animals. PNAS, 112:8874-8878, 2015.
KIM, T.; LEIBFRIED-RUTLEDGE, M.L.; FIRST, N.L. Gene transfer in bovine blastocysts using replication-defective retroviral vectors packaged with Gibbon ape leukemia virus envelopes. Mol Reprod Dev, 35: 105-113, 1993.
KIM, G.A.; LEE, E.M.; JIN, J.X.; LEE, S.; TAWEECHAIPAISANKUL, A.; HWANG, J.I.; ALAM, Z.; AHN, C.; LEE, B.C. Generation of MAHKO/GTKO/shTNFRI-Fc/HO-1 quadruple gene modified pigs. Transgenic Res, 26: 435-445, 2017.
KIMURA, Y.; YANAGIMACHI, R. Intracytoplasmic sperm injection in the mouse. Biol Reprod, 52: 709-720, 1995.
KOLLER, B.H.; HAGEMANN, L.J.; DOETSCHMAN, T.; HAGAMAN, J.R.; HUANG, S.; WILLIAMS, P.J.; FIRST, N.L.; MAEDA, N.; SMITHIES, O. Germ-line transmission of a planned alteration made in a hypoxanthine phosphoribosyltransferase gene by homologous recombination in embryonic stem cells. PNAS, 86: 8927-8931, 1989.
KRAUSE, M.N.; SANCHO-MARTINEZ, I.; IZPISUA BELMONTE, J.C. Understanding the molecular mechanisms of reprogramming. Biochemical and Biophysical Research Communications, 473: 693-697, 2016.
KUROIWA, Y.; KASINATHAN, P.; CHOI, Y.J.; NAEEM, R.; TOMIZUKA, K.; SULLIVAN, E.J.; KNOTT, J.G.; DUTEAU, A.; GOLDSBY, R.A.; OSBORNE, B.A.; ISHIDA, I.; ROBL, J.M. Cloned transchromosomic calves producing human immunoglobulin. Nat Biotech, 20: 889-894, 2002.
KUROIWA, Y.; KASINATHAN, P.; MATSUSHITA, H.; SATHIYASELAN, J.; SULLIVAN, E.J.; KAKITANI, M.; TOMIZUKA, K.; ISHIDA, I.; ROBL, J.M. Sequential targeting of the genes encoding immunoglobulin-mu and prion protein in cattle. Nat Genet, 36: 775-780, 2004.
LAI, L.; KOLBER-SIMONDS, D.; PARK, K.W.; CHEONG, H.T.; GREENSTEIN, J.L.; IM, G.S.; SAMUEL, M.; BONK, A.; RIEKE, A.; DAY, B.N.; MURPHY, C.N.; CARTER, D.B.; HAWLEY, R.J.; PRATHER, R.S. Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science, 295: 1089-1092, 2002.
LARSON, G.; BURGER, J. A population genetics view of animal domestication. Trends Genet, 29: 197-205, 2013.
LAVITRANO, M.; CAMAIONI, A.; FAZIO, V.M.; DOLCI, S.; FARACE, M.G.; SPADAFORA, C. Cell, 57: 717-723, 1989.
LAVITRANO, M.; BUSNELLI, M.; CERRITO, M.G.; GIOVANNONI, R.; MANZINI, S.; VARGIOLU, A. Sperm-mediated gene transfer. Reprod Fertil Dev, 18: 19-23, 2006.
LISAUSKAS, S.F.; RECH, E.L.; ARAGÃO, F.J. Characterization of transgene integration loci in transformed Madin Darby bovine kidney cells. Cloning Stem Cells, 9: 456-460, 2007.
LOI, P.; IUSO, D.; CZERNIK, M.; OGURA, A. A New, Dynamic Era for Somatic Cell Nuclear Transfer? Trends Biotech, 34: 791-797, 2016.
LONERGAN, P.; FAIR, T. Maturation of Oocytes in Vitro. Annu Rev Anim Biosci, 4: 255-268, 2016.
LóPEZ-SAUCEDO, J.; PARAMIO-NIETO, M.T.; FIERRO, R.; PIñA-AGUILAR, R.E. Intracytoplasmic sperm injection (ICSI) in small ruminants. Anim Reprod Sci, 133: 129- 138, 2012.
LOTTI, S.N.; POLKOFF, K.M.; RUBESSA, M.; WHEELER, M.B. Modification of the Genome of Domestic Animals. Anim Biotechnol, 28: 198-210, 2017.
LUSH, J.L.The impact of genetics on animal breeding. J Anim Sci, 10: 311-321, 1951.
MARTIN, G.R. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. PNAS, 78: 7634-7638, 1981.
MCCREATH, K.J.; HOWCROFT, J.; CAMPBELL, K.H.; COLMAN, A.; SCHNIEKE, A.E.; KIND, A.J. Production of gene-targeted sheep by nuclear transfer from cultured somatic cells. Nature, 405: 1066-1069, 2000.
MELO, E.O.; CANAVESSI, A.M.; FRANCO, M.M.; RUMPF, R. Animal transgenesis: state of the art and applications. J Appl Genet, 48: 47-61, 2007.
MEUWISSEN, T.H.; HAYES, B.J.; GODDARD, M.E. Prediction of total genetic value using genome-wide dense marker maps. Genetics, 157: 1819-1829, 2001.
MOGHADDASSI, S.; EYESTONE, W.; BISHOP, C.E. TALEN-mediated modification of the bovine genome for large-scale production of human serum albumin. PLoS One, 9: e89631, 2014.
MOISYADI, S.; KAMINSKI, J.M.; YANAGIMACHI, R. Use of intracytoplasmic sperm injection (ICSI) to generate transgenic animals. Comp Immunol Microbiol Infect Dis, 32: 47-60, 2009.
MOURA, M.T. Pluripotency and cellular reprogramming. Anais da Academia Pernambucana de Ciência Agronômica, 8: 138-168, 2012.
NAGY, A.; GóCZA, E.; DIAZ, E.M.; PRIDEAUX, V.R.; IVÁNYI, E.; MARKKULA, M.; ROSSANT, J. Embryonic stem cells alone are able to support fetal development in the mouse. Development, 110: 815-821, 1990.
NAGY, A.; ROSSANT, J.; NAGY, R.; ABRAMOW-NEWERLY, W.; RODER, J.C. Derivation of completely cell culture-derived mice from early-passage embryonic stem cells. PNAS, 90: 8424-8428, 1993.
NI, W.; QIAO, J.; HU, S.; ZHAO, X.; REGOUSKI, M.; YANG, M.; POLEJAEVA, I.A.; CHEN, C. Efficient gene knockout in goats using CRISPR/Cas9 system. PLoS One, 9:e106718, 2014.
NIEMANN, H.; KUES, W.A. Application of transgenesis in livestock for agriculture and biomedicine. Anim Reprod Sci, 79: 291-317, 2003.
NIU, Y.; LIANG, S. Progress in gene transfer by germ cells in mammals. J Genet Genomics, 35: 701-714, 2008.
OGOREVC, J.; OREHEK, S.; DOVč, P. Cellular reprogramming in farm animals: an overview of iPSC generation in the mammalian farm animal species. J Anim Sci Biotech, 7: 10, 2016.
OKITA, K.; ICHISAKA, T.; YAMANAKA, S. Generation of germline–competent induced pluripotent stem cells. Nature, 448: 313-317, 2007.
PARAMIO, M.T.; IZQUIERDO, D. Current status of in vitro embryo production in sheep and goats. Reprod Domest Anim, 49 (Supplement 4): 37-48, 2014.
PARK, F. Lentiviral vectors: are they the future of animal transgenesis? Physiol Genomics.,31: 159-73, 2007.
PEREYRA-BONNET, F.; FERNÁNDEZ-MARTÍN, R.; OLIVERA, R.; JARAZO, J.;VICHERA, G.; GIBBONS, A.; SALAMONE, D. A unique method to produce transgenic embryos in ovine, porcine, feline, bovine and equine species. Reprod Fert Dev, 20: 741-749, 2008.
PERRY, A.C.; WAKAYAMA, T.; KISHIKAWA, H.; KASAI, T.; OKABE, M.; TOYODA,Y.; YANAGIMACHI, R. Mammalian transgenesis by intracytoplasmic sperm injection.Science, 284: 1180-1183, 1999.
PERRY, A.C.; ROTHMAN, A.; DE LAS HERAS, J.I.; FEINSTEIN, P.; MOMBAERTS, P.;COOKE, H.J.; WAKAYAMA, T. Efficient metaphase II transgenesis with different transgene archetypes. Nat Biotechnol, 19: 1071-1073, 2001.
PETERSEN, B.; NIEMANN, H. Molecular scissors and their application in genetically modified farm animals. Transgenic Res, 24: 381-396, 2015.
PHELPS, C.J.; KOIKE, C.; VAUGHT, T.D.; BOONE, J.; WELLS, K.D.; CHEN, S.H.;BALL, S.; SPECHT, S.M.; POLEJAEVA, I.A.; MONAHAN, J.A.; JOBST, P.M.; SHARMA,S.B.; LAMBORN, A.E.; GARST, A.S.; MOORE, M.; DEMETRIS, A.J.; RUDERT, W.A.;BOTTINO, R.; BERTERA, S.; TRUCCO, M.; STARZL, T.E.; DAI, Y.; AYARES, D.L.Production of alpha 1,3-galactosyltransferase-deficient pigs. Science, 299: 411-414, 2003.
POLEJAEVA, I.A.; RUTIGLIANO, H.M.; WELLS, K.D. Livestock in biomedical research: history, current status and future prospective. Reprod Fertil Dev, 28: 112-124, 2016.
RAZZA, E.M.; SATRAPA, R.A.; EMANUELLI, I.P.; BARROS, C.M.; NOGUEIRA, M.F. Screening of biotechnical parameters for production of bovine inter-subspecies embryonic chimeras by the aggregation of tetraploid Bos indicus and diploid crossbred Bos taurus embryos. Reprod Biol, 16: 34-40, 2016.
ROGERS, C.S. Genetically engineered livestock for biomedical models. Transgenic Res,25: 345-359, 2016.
SAITO, S.; SAWAI, K.; UGAI, H.; MORIYASU, S.; MINAMIHASHI, A.; YAMAMOTO, Y.; HIRAYAMA, H.; KAGEYAMA, S.; PAN, J.; MURATA, T.; KOBAYASHI, Y.; OBATA,Y.; YOKOYAMA, K.K. Generation of cloned calves and transgenic chimeric embryos from bovine embryonic stem-like cells. Biochem Biophys Res Commun, 309: 104-113, 2003.
SATO, M.; OHTSUKA, M.; WATANABE, S.; GURUMURTHY, C.B. Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new. Biol Direct, 11: 16, 2016.
SCHNIEKE, A.E.; KIND, A.J.; RITCHIE, W.A.; MYCOCK, K.; SCOTT, A.R.; RITCHIE,M.; WILMUT, I.; COLMAN, A.; CAMPBELL, K.H. Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts. Science, 278: 2130-2133, 1997.
SILVA, P.G.C.; MOURA, M.T.; BRAGA, V.A.A.; FERREIRA-SILVA, J.C.; NASCIMENTO, P.S.; CANTANHêDE, L.F.; CHAVES, M.S.; OLIVEIRA, M.A.L. Atividade dos genes relacionados à pluripotência em ovinos. Med Vet (UFRPE), v, 2017.
SMITH, K.R. Sperm cell mediated transgenesis: a review. Anim Biotech, 10: 1-13, 1999.
SMITH, A.G. Embryo–derived stem cells: of mice and men. Annu Rev Cell Dev Biol, 17:435–462, 2001.
SOTO, D.A.; ROSS, P.J. Pluripotent stem cells and livestock genetic engineering. Transgenic Res, 25: 289-306, 2016.
SQUIRE, K.R.; EMBRETSON, J.E.; FIRST, N.L. In vitro testing of a potential retroviral vector for producing transgenic livestock. Am J Vet Res, 50: 1423-1427, 1989.
TAKAHASHI, K.; YAMANAKA, S. A developmental framework for induced pluripotency.Development, 142: 3274-3285, 2015.
TAN, W.; CARLSON, D.F.; LANCTO, C.A.; GARBE, J.R.; WEBSTER, D.A.; HACKETT,P.B.; FAHRENKRUG, S.C. Efficient nonmeiotic allele introgression in livestock using custom endonucleases. PNAS, 110: 16526-16531, 2013.
TANG, L.; GONZÁLEZ, R.; DOBRINSKI, I. Germline modification of domestic animals. Anim Reprod, 12: 93-104, 2015.
TANIHARA, F.; TAKEMOTO, T.; KITAGAWA, E.; RAO, S.; DO, L.T.; ONISHI, A.; YAMASHITA, Y.; KOSUGI, C.; SUZUKI, H.; SEMBON, S.; SUZUKI, S.; NAKAI, M.; HASHIMOTO, M.; YASUE, A.; MATSUHISA, M.; NOJI, S.; FUJIMURA, T.;FUCHIMOTO, D.; OTOI, T. Somatic cell reprogramming-free generation of genetically modified pigs. Sci Adv, 2: e1600803, 2016.
TOMINAGA, K.; OLGUN, A.; SMITH, J.R.; PEREIRA-SMITH, O.M. Genetics of cellular senescence. Mech Ageing Dev, 123: 927-936, 2002.
VERMA, V.; HUANG, B.; KALLINGAPPA, P.K.; OBACK, B. Dual kinase inhibition promotes pluripotency in finite bovine embryonic cell lines. Stem Cells Dev, 22: 1728-1742, 2013.
WALL, R.J. Pronuclear microinjection. Cloning Stem Cells, 3: 209-220, 2001. WALL, R.J. New gene transfer methods. Theriogenology, 57: 189-201, 2002.
WALL, R.J. Transgenic livestock: progress and prospects for the future. Theriogenology,45: 57-68, 1996.
WANG, G.D.; XIE, H.B.; PENG, M.S.; IRWIN, D.; ZHANG, Y.P. Domestication genomics: evidence from animals. Annu Rev Anim Biosci, 2: 65-84, 2014.
WANG, Z. Genome engineering in cattle: recent technological advancements. Chromosome Res., 23: 17-29, 2015.
WEISS, E.H.; LILIENFELD, B.G.; MÜLLER, S.; MÜLLER, E.; HERBACH, N.; KESSLER, B.; WANKE, R.; SCHWINZER, R.; SEEBACH, J.D.; WOLF, E.; BREM, G. HLA-E/human beta2-microglobulin transgenic pigs: protection against xenogeneic human anti-pig natural killer cell cytotoxicity. Transplantation, 87: 35-43, 2009.
WERNIG, M.; MEISSNER, A.; FOREMAN, R.; BRAMBRINK, T.; KU, M.;
HOCHEDLINGER, K.; BERNSTEIN, B.E.; JAENISCH R. In vitro reprogramming of broblasts into a pluripotent ES–cell–like state. Nature, 448: 318-324, 2007.
WHEELER, M.B. Development and validation of swine embryonic stem cells: a review. Reprod Fertil Dev, 6: 563-568, 1994.
WIENER, P.; WILKINSON, S. Deciphering the genetic basis of animal domestication. Proc Biol Sci, 278: 3161-3170.,2011.
WOBUS, A.M.; BOHELER, K.R. Embryonic stem cells: prospects for developmental biology and cell therapy. Phys Rev, 85: 635-678, 2005.
WU, H.; WANG, Y.; ZHANG, Y.; YANG, M.; LV, J.; LIU, J.; ZHANG, Y. TALE nickase-mediated SP110 knockin endows cattle with increased resistance to tuberculosis. PNAS, 112:E1530-1539, 2015.
WU, J.; PLATERO-LUENGO, A.; SAKURAI, M.; SUGAWARA, A.; GIL, M.A.; YAMAUCHI, T.; SUZUKI, K.; BOGLIOTTI, Y.S.; CUELLO, C.; MORALES VALENCIA, M.; OKUMURA, D.; LUO, J.; VILARIñO, M.; PARRILLA, I.; SOTO, D.A.; MARTINEZ, C.A.; HISHIDA, T.; SÁNCHEZ-BAUTISTA, S.; MARTINEZ-MARTINEZ, M.L.; WANG, H.; NOHALEZ, A.; AIZAWA, E.; MARTINEZ-REDONDO, P.; OCAMPO, A.; REDDY, P.; ROCA, J.; MAGA, E.A.; ESTEBAN, C.R.; BERGGREN, W.T.; NUñEZ DELICADO, E.; LAJARA, J.; GUILLEN, I.; GUILLEN, P.; CAMPISTOL, J.M.; MARTINEZ, E.A.; ROSS, P.J.; IZPISUA BELMONTE, J.C. Interspecies Chimerism with Mammalian Pluripotent Stem Cells. Cell, 168: 473-486, 2017.
YAMANAKA, S.; BLAU, H.M. Nuclear reprogramming to a pluripotent state by three approaches. Nature, 465: 704-712, 2010.
ZHOU, Z.R.; ZHONG, B.S.; JIA, R.X.; WAN, Y.J.; ZHANG, Y.L.; FAN, Y.X.; WANG, L.Z.; YOU, J.H.; WANG, Z.Y.; WANG, F. Production of myostatin-targeted goat by nuclear transfer from cultured adult somatic cells. Theriogenology, 79: 225-233, 2013.
