Successful Tulasnella amonilioides isolation from wild Cattleya intermedia and effectiveness of the mycobiont on in vitro propagation of this threatened Orchidaceae

Autores

  • Delio Endres Júnior Universidade Feevale
  • Genivaldo Alves-Silva Universidade Federal do Rio Grande do sul
  • Márcio Hisayuki Sasamori Universidade Feevale
  • Rosa Mara Borges da Silveira Universidade Federal do Rio Grande do sul
  • Annette Droste Universidade Feevale

DOI:

https://doi.org/10.24221/jeap.8.1.2023.5160.009-029

Palavras-chave:

Epiphytic orchid, Epulorhiza sp., orchid conservation, phylogenetic analysis, symbiosis

Resumo

This is the first study that reports symbiosis in Cattleya, aiming to isolate and identify mycorrhizal fungi capable of promoting the germination of this orchid and to evaluate the development of symbiotically propagated individuals. We compared seed germination percentage, growth index, and morphometric variables of seedlings propagated symbiotically in oatmeal agar (OMA) medium with individuals that were non-symbiotically propagated in Murashige and Skoog (MS) medium. Fungi isolates were identified by phylogenetic analysis and eight of the nine isolates that were efficient in C. intermedia propagation were identified as Tulasnella amonilioides. The mycobiont improved C. intermedia seed germination and plant development when compared with OMA medium without fungi (negative control). Seedlings propagated by symbiotic culture with T. amonilioides produced more leaves and longer roots, while shoot height and a number of roots were lower than for seedlings propagated in MS medium with the addition of activated charcoal. The fresh mass of seedlings propagated by symbiotic and a symbiotic techniques was equal, except when seedlings were grown in MS without activated charcoal. T. amonilioides enhance the in vitro propagation of C. intermedia and provide plants that facilitate symbiotic processes in reintroduction environments.

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Biografia do Autor

Delio Endres Júnior, Universidade Feevale

Doutor em Qualidade Ambiental, Pós-doutorando do programa de Pós-graduação em Qualidade Ambiental, Universidade Feevale. 

Genivaldo Alves-Silva, Universidade Federal do Rio Grande do sul

Doutor em Botânica, Universidade Federal do Rio Grande do Sul. Pós-doutorando do Programa de Pós-Graduação em Biologia de Fungos, Algas e Plantas, Universidade Federal de Santa Catarina 

Márcio Hisayuki Sasamori, Universidade Feevale

Doutor em Qualidade Ambiental, Programa de Pós-graduação em Qualidade Ambiental, Universidade Feevale.

Rosa Mara Borges da Silveira, Universidade Federal do Rio Grande do sul

Doutora em Ciências Biológicas pelo Universidad de Buenos Aires, Argentina, Professora titular do Programa de Pós-Graduação em Botânica, Universidade Federal do Rio Grande do Sul.

Annette Droste, Universidade Feevale

Doutora em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul. Professora titular do Programa de Pós-graduação em Qualidade Ambiental da Universidade Feevale. 

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2023-03-06

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Endres Júnior, D., Alves-Silva, G. ., Sasamori, M. H., Silveira, R. M. B. da, & Droste, A. (2023). Successful Tulasnella amonilioides isolation from wild Cattleya intermedia and effectiveness of the mycobiont on in vitro propagation of this threatened Orchidaceae. Journal of Environmental Analysis and Progress, 8(1), 009–029. https://doi.org/10.24221/jeap.8.1.2023.5160.009-029

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v. 8 n. 1 (2023): Journal of Environmental Analysis and Progress

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Copyright (c) 2023 Delio Endres Júnior, Genivaldo Alves-Silva, Márcio Hisayuki Sasamori, Rosa Mara Borges da Silveira, Annette Droste

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