Vaginal microbiota of nulliparous and multiparous cows and their resistance to antimicrobials

Autores

  • Lucas Pereira Silva Editor-Gerente Universidade Federal Rural de Pernambuco - UFRPE
  • Sebastião Ávila Ramos Regional Council of Veterinary Medicine, Alagoas-AL, Brazil.
  • Cícero Cerqueira Cavalcanti Neto Microbiology Laboratory, Center of Agricultural Sciences, Federal University of Alagoas, Alagoas-AL, Brazil.
  • Tania Marta Carvalho dos Santos Microbiology Laboratory, Center of Agricultural Sciences, Federal University of Alagoas, Alagoas-AL, Brazil.
  • Jorge Alberto Cavalcante de Oliveira Microbiology Laboratory, Center of Agricultural Sciences, Federal University of Alagoas, Alagoas-AL, Brazil.
  • Micheline Thais dos Santos Microbiology Laboratory, Center of Agricultural Sciences, Federal University of Alagoas, Alagoas-AL, Brazil.
  • João Manoel da Silva Microbiology Laboratory, Center of Agricultural Sciences, Federal University of Alagoas, Alagoas-AL, Brazil.
  • José Soares Brito Neto Microbiology Laboratory, Center of Agricultural Sciences, Federal University of Alagoas, Alagoas-AL, Brazil.
  • Yamina Coentro Montaldo Microbiology Laboratory, Center of Agricultural Sciences, Federal University of Alagoas, Alagoas-AL, Brazil.

DOI:

https://doi.org/10.26605/medvet-v13n3-3315

Palavras-chave:

Enterococcus, Staphylococcus aureus, Escherichia coli, Candida sp., antimicrobials

Resumo

Infertility and prenatal mortality are the most important causes of production losses in domestic animals. The presence of pathogenic agents in the female reproductive system of cattle can negatively influence the reproductive performance of the herd. The objective of this study was to isolate, quantify, and characterize the components of the vaginal microbiota of multiparous and nulliparous mongrel cows, focusing on proper health and reproductive management. Samples were collected from 20 healthy animals: 10 nulliparous and 10 multiparous cows. The inoculation was performed simultaneously on four different culture media: BBL CHROM agar Candida, BBL Bile Esculin Agar Slants, Baird-Parker Agar, and MacConkey Agar bile salts. The isolated microorganisms were identified according to morphological and biochemical features. Microorganisms were detected in 80% of the nulliparous and 10% of the multiparous cows, with the following frequency: 88.78% of bacteria had morphophysiological characteristics of Staphylococcus spp., 11.22% of Escherichia coli, and 120 isolated yeast colonies which were identified as Candida tropicalis (69%), C. albicans (24%), and C. krusei. Seventeen isolates of Staphylococcus spp. (53.12%) presented sensitivity to all antimicrobials tested: 65.6% to amoxillin, 46.9% to erythromycin, 71.8% to rifampicin, and 46.9% to tetracycline. The rate of resistance to antimicrobials (MDR) was 0.125. The presence of typical microorganisms was detected. The MDR indicated that the isolates did not show multiresistance, with only two (6.25%) resistant to more than one antimicrobial simultaneously. The bacterial isolates studied were sensitive to the antimicrobial drugs tested, demonstrating the potential for use of these active ingredients.

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Publicado

23-04-2020

Como Citar

Silva, L. P., Ramos, S. Ávila, Cavalcanti Neto, C. C., dos Santos, T. M. C., de Oliveira, J. A. C., dos Santos, M. T., da Silva, J. M., Brito Neto, J. S., & Montaldo, Y. C. (2020). Vaginal microbiota of nulliparous and multiparous cows and their resistance to antimicrobials. Medicina Veterinária, 13(3), 474–481. https://doi.org/10.26605/medvet-v13n3-3315

Edição

v. 13 n. 3 (2019)

Seção

Reprodução Animal

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