Lactoperoxidase enzyme activity and thiocyanate levels in raw milk of Girolando cows
DOI:
https://doi.org/10.26605/medvet-v14n4-2544Palavras-chave:
somatic cells, bovine milk, lactoperoxidase system.Resumo
Considering the importance of milk enzymes in milk preservation and animal protection, this study aimed to identify the factors that influence lactoperoxidase activity and levels of thiocyanate in raw milk of Girolando cows, as well as to verify the correlation between these components and the number of somatic cells in cooled raw milk. A total of 181 of milk samples from Girolando cows were used to determine the lactoperoxidase activity, thiocyanate levels, somatic cell number, and serum protein content. The genetic group and the sampling period influenced lactoperoxidase activity. Thiocyanate levels were influenced by the sampling period and by the interaction between the genetic group and the sampling period. The number of somatic cells was influenced by the number of days in lactation, animal age, and sampling period. Milk of animals from the group 1/2 Holstein x Gyr had the highest lactoperoxidase activity and there was no influence of the genetic group on the levels of thiocyanate.Downloads
Referências
Al-Baarri, A.N.; Ogawa, M.; Visalsok, T.; Hayakawa, S. Lactoperoxidase immobilized onto various beads for producing natural preservatives solution. Journal of Applied Food Technology, 1(1): 4-6, 2012.
Althaus, R.L.; Molina, M.P.; Rodríguez, M.; Fernández, N. Analysis time and lactation stage influence on lactoperoxidase system components in dairy ewe milk. Journal of Dairy Science, 84(8): 1829-1835, 2001.
Andrews, A.T.; Olivecrona, T.; Begtsson-Olivecrona, G.; Fox, P.F.; Bjorck, L.; Farkye, N.Y. Indigenous enzymes in milk. In: Fox, P.F. Food Enzymology. 1st ed. London: Elsevier, 1991. p.53-129.
Asadpour, R.; Tayefi-Nasrabadi, H.; Moghadam, G.A.; Nofouzi, K. Correlation between lactoperoxidase activity and somatic cell count for diagnosis subclinical mastitis in early lactation of dairy cows. Journal of Animal and Veterinary Advances, 7(7): 777-779, 2008.
Atasever, A.; Ozdemir, H.; Gulcin, I.; Kufrevioglu, O.I. One-step purification of lactoperoxidase from bovine milk by affinity chromatography. Food Chemistry, 136: 864-870, 2013.
Boots, J.W.; Floris, R. Lactoperoxidase: from catalytic mechanism to practical applications. International Dairy Journal, 16: 1272-1276, 2006.
CAC. Codex Alimentarius Commission. Guidelines for the preservation of raw milk by use of the lactoperoxidase system, CAC/GL 13-1991, 1991. Available at: . Accessed on: 01 dec 2018.
Calamari, L.; Maianti, M.G.; Bani, P.; Sarti, L. Seasonal variations of some enzyme activities of cow milk. Italian Journal of Animal Science, 4: 212-214, 2005.
Fonteh, F.A.; Grandison, A.S.; Lewis, M.J. Variations of lactoperoxidase activity and thiocyanate content in cows and goats milk throughout lactation. Journal of Dairy Research, 69: 401-409, 2002.
Fweja, L.W.; Lewis, M.J.; Grandison, A.S. Alternative strategies for activation of the natural lactoperoxidase system in cows' milk: trials in Tanzania. Journal of Dairy Research, 74(4): 381-386, 2007.
Isobe, N.; Kubota, H.; Yamasaki, A.; Yoshimura, Y. Lactoperoxidase activity in milk is correlated with somatic cell count in dairy cows. Journal of Dairy Science, 94(8): 3868-3874, 2011.
Korhonen, H. A new method for preserving milk - the lactoperoxidase antibacterial system. World Animal Review, 35: 23-29, 1980.
Kumar, R.; Bhatia, K.L. Standardization of method for lactoperoxidase assay in milk. Lait, 79(2): 269-274, 1999.
Kussendrager, K.D.; Van Hooijdonk, A.C.M. Lactoperoxidase: physico-chemical properties, occurence, mechanism of action and application. British Journal of Nutrition, 84: S19-S25, 2000.
Lujerdean, A.; Bunea, A. The influence of lactation stage on lactoperoxidase activity. Animal Science and Biotechnologies, 67(1-2): 231-234, 2010.
Ponce, P.; Alfonso, H.A.; Diaz, B.; Capdevila, J.; Yanez, J. Factores asociados al contenido de tiocianato en leche cruda. Revista de Salud Animal, 76: 96-99, 1998.
Ponce, P. Thiocyanate content in raw milk under the American tropic conditions in relation to the activation of the lactoperoxidase system. Revista de Salud Animal, 34(2): 115-119, 2012.
Seifu, E.; Buys, E.M.; Donkin, E.F. Significance of lactoperoxidase system in the dairy industry and its potential applications: a review. Food Science and Technology, 16: 137-157, 2005.
Seifu, E.; Donkin, E.F.; Buys, E.M. Potential of lactoperoxidase to diagnose subclinical mastitis in goats. Small Ruminant Research, 69: 154-158, 2007.
Sermon, J.; Vanoirbeek, K.; Spiegeleer, P. de.; Van Houdt, R.; Aertsen, A.; Michiels, C.W. Unique stress response to the lactoperoxidase-thiocyanate enzyme system in Escherichia coli. Reseach in Microbiolology, 156(2): 225-232, 2005.
Shook, G.E.; Schutz, M.M. Selection on somatic cell score to improve resistance to mastitis in the United States. Journal of Dairy Science, 77: 648-658, 1994.
Yaqub, T.; Sadaf, S.; Mukhtar, N.; Zaneb, H.; Shabbir, M.Z.; Ahmad, A.; Aslam, A.; Ashraf, K.; Ahmad, N.; Rehman, H. The influence of time elapsed after milking and lactation stage on the lactoperoxidase system of milk in Sahiwal cattle and Nili-Ravi buffaloes. International Journal of Dairy Technology, 65(3): 360-364, 2012.
Downloads
Publicado
Como Citar
Edição
Seção
Licença
- A Revista de Medicina Veterinária permite que o autor retenha os direitos de publicação sem restrições, utilizando para tal a licença Creative Commons CC BY-NC-SA 4.0.
- De acordo com os termos seguintes:
- Atribuição — Você deve dar o crédito apropriado, prover um link para a licença e indicar se mudanças foram feitas. Você deve fazê-lo em qualquer circunstância razoável, mas de nenhuma maneira que sugira que o licenciante apoia você ou o seu uso.
- NãoComercial — Você não pode usar o material para fins comerciais.
- CompartilhaIgual — Se você remixar, transformar, ou criar a partir do material, tem de distribuir as suas contribuições sob a mesma licença que o original.
- Sem restrições adicionais — Você não pode aplicar termos jurídicos ou medidas de caráter tecnológico que restrinjam legalmente outros de fazerem algo que a licença permita.