Automated impedance-based and manual leukocyte differential counts in healthy equines

Autores

  • Maria Fernanda Cazini Silva Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil https://orcid.org/0009-0003-5300-0987
  • Luma Maria Rovina Brito Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil https://orcid.org/0009-0005-4836-6540
  • Beatriz de Azevedo Martins Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil https://orcid.org/0000-0002-5746-4303
  • Laryssa Eduarda de Campos Lopes Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil https://orcid.org/0000-0002-0119-1043
  • Yasmin Cunha Duarte Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil https://orcid.org/0000-0002-0015-6481
  • Matheus Nogueira Morais Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil https://orcid.org/0000-0002-7106-2538
  • Laura Arduino Vasconcelos Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil https://orcid.org/0000-0001-5542-6656
  • Mariana Orlandini Mendonça Ourinhos Veterinary Hospital (HVO), Ourinhos-SP, Brazil https://orcid.org/0000-0001-8394-0521
  • Tainara de Oliveira Martins Canguru Veterinary Hospital, Ourinhos-SP, Brazil https://orcid.org/0009-0005-4684-3446
  • Daniela Fernandez Montechiesi Autonomous Veterinary, Ourinhos-SP, Brazil https://orcid.org/0009-0000-0419-3303
  • Breno Fernando Martins de Almeida Department of Veterinary Medicine, Roque Quagliato Veterinary Hospital, University Center of the Integrated Faculties of Ourinhos (Unifio), Ourinhos-SP, Brazil. Faculty of Veterinary Medicine of Araçatuba, São Paulo State University, (FMVA-Unesp), Araçatuba-SP, Brazil https://orcid.org/0000-0002-6379-6734

DOI:

https://doi.org/10.26605/medvet-v17n4-5897

Palavras-chave:

horse, leukogram, impedance

Resumo

The speed and convenience provided by automated cell counters are reasons for the increasing use of this technology in veterinary practice, with impedance methodology being one of the most commonly used in Brazil. In this regard, the objective of this study was to compare the automated differential leukocyte count obtained by impedance with the manual count obtained from the evaluation of blood smears in healthy horses. For this purpose, the automated differential leukocyte count by impedance using a veterinary cell counter (Abx Micros ESV 60, Horiba) was compared to the manual count using optical microscopy in 545 blood samples from healthy horses. Paired t-tests or Wilcoxon tests, Deming regression, Pearson or Spearman correlations, and Bland-Altman analysis were used, considering differences significant when p<0.05. All optical microscopy counts were carried out by the same veterinary clinical pathologist with ten years of previous experience in the area. The automated method showed higher concentrations of segmented neutrophils and monocytes, and lower concentrations of lymphocytes and eosinophils compared to the manual method. The mean error of the automated count was -10.26% for segmented neutrophils, 11.04% for lymphocytes, -41.39% for monocytes, and -10.84% for eosinophils, ranging from -200% to 161.4% depending on the cell type. There was a significant correlation between the methodologies only for the counts of segmented neutrophils, lymphocytes, and eosinophils. In conclusion, the manual differential leukocyte count in healthy horses cannot be replaced by the automated method, and the analysis of blood smears remains a fundamental tool for the proper interpretation of the leukogram in horses.

Downloads

Não há dados estatísticos.

Referências

Bauer, N.; Nakagawa, J.; Dunker, C.; Failing, K.; Moritz, A. Evaluation of the automated hematology analyzer Sysmex XT-2000iV™ compared to the ADVIA® 2120 for its use in dogs, cats, and horses. Part II: accuracy of leukocyte differential and reticulocyte count, impact of anticoagulant and sample aging. Journal of Veterinary Diagnostic Investigation, 24(1): 74-89, 2012.

Borges, L.F.; Siqueira, L.O. Validação de tecnologia 5diff do analisador hematológico Sysmex XS-1000i para laboratório de pequeno/médio porte. Revista Brasileira de Hematologia e Hemoterapia, 31(4): 247-251, 2009.

DeNicola, D.B. Advances in hematology analyzers. Topics in Companion Animal Medicine, 26: 52-61, 2011.

Freeman, K.P.; Farr, A.J.; Barrelet, A. Hematology of equids. In: Brooks, M.B.; Harr, K.E.; Seelig, D.M.; Wardrop, K.J.; Weiss, D.J. Schalm’s Veterinary Hematology. 7th ed. Ames: Wiley-Blackwell, 2022. Cap.110, p.993-1003.

Giordano A.; Rossi G.; Pieralisi C.; Paltrinieri S. Evaluation of equine hemograms using the ADVIA 120 as compared with an impedance counter and manual differential count. Veterinary Clinical Pathology, 37(1): 21-30, 2008.

González, F.H.D.; Silva, S.C.; Patologia clínica veterinária: texto introdutório. Porto Alegre: Universidade Federal do Rio Grande do Sul, 2008. 342 p.

Grotto, H.Z.W. O hemograma: importância para a interpretação da biópsia. Revista Brasileira de Hematologia e Hemoterapia, 31(3): 178-182, 2009.

Jain, N.C. Hematologic techniques. In: Jain, N.C. Schalm´s Veterinary Hematology. 4th ed. Philadelphia: Lea & Febiger, 1986. p.20-86.

Lilliehöök, I.; Tvedten, H. Validation of the Sysmex XT-2000iV hematology system for dogs, cats, and horses. I. Erythrocytes, platelets, and total leukocyte counts. Veterinary Clinical Pathology, 38(2): 163-174, 2009a.

Lilliehöök, I.; Tvedten, H. Validation of the Sysmex XT?2000iV hematology system for dogs, cats, and horses. II. Differential leukocyte counts. Veterinary Clinical Pathology, 38: 175-182, 2009b.

Miranda, G.M.D. Validação do analisador hematológico automático BC-2800 VET® para realização de hemogramas de muares. 2014. Dissertação (Mestrado). Ciência Animal, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Araçatuba, 2014. 35p.

Moritz, A.; Fickenscher, Y.; Meyer, K.; Failing, K.; Weiss, D.J. Canine and feline hematology reference values for the ADVIA 120 hematology system. Veterinary Clinical Pathology, 33(1): 32-38, 2004.

Nabity, M.B.; Harr, K.E.; Camus, M.S.; Flatland, B.; Vap, L.M. ASVCP guidelines: allowable total error hematology. Veterinary Clinical Pathology, 47(1): 1-13, 2018.

Perez-Ecija, A.; Gonzalez?De Cara, C.A.; Aguilera?Aguilera, R.; Estepa, J.C.; Rubio, M.D.; Mendoza, F.J. Comparison of donkey hemogram using the LaserCyte hematology analyzer, an impedance system, and a manual method. Veterinary Clinical Pathology, 43: 525-537, 2014.

Riond, B.; Weissenbacher, S.; Hofmann-Lehmann, R.; Lutz, H. Performance evaluation of the Sysmex pocH-100iV Diff hematology analyzer for analysis of canine, feline, equine, and bovine blood. Veterinary Clinical Pathology, 40(4): 484-495, 2011.

Siqueira, V.C.; Bastos, P.A.S. Bem-Estar Animal para clínicos veterinários. Brazilian Journal Health Review, 3(2): 1713-1746, 2020.

Souza, R.F.P.; Bosco, A.M.; Pereira, P.P.; Narciso, L.G.; Almeida, B.F.M. Contagem diferencial de leucócitos automatizada e manual em cães. Revista CFMV, 1(87): 51-61, 2021.

Welles, E.G.; Hall, A.S.; Carpenter, D.M. Canine complete blood counts: a comparison of four in-office instruments with the ADVIA 120 and manual differential counts. Veterinary Clinical Pathology, 38(1): 20-29, 2009.

Downloads

Publicado

28-12-2023

Como Citar

Silva , M. F. C., Brito, L. M. R., Martins, B. de A., Lopes, L. E. de C., Duarte, Y. C., Morais, M. N., Vasconcelos, L. A., Mendonça, M. O., Martins, T. de O., Montechiesi, D. F., & Martins de Almeida, B. F. (2023). Automated impedance-based and manual leukocyte differential counts in healthy equines. Medicina Veterinária, 17(4), 249–256. https://doi.org/10.26605/medvet-v17n4-5897

Edição

v. 17 n. 4 (2023): Medicina Veterinária

Seção

Patologia Animal

Licença

Copyright (c) 2023 Maria Fernanda Cazini Silva , Luma Maria Rovina Brito, Beatriz de Azevedo Martins, Laryssa Eduarda de Campos Lopes, Yasmin Cunha Duarte, Matheus Nogueira Morais, Laura Arduino Vasconcelos, Mariana Orlandini Mendonça, Tainara de Oliveira Martins, Daniela Fernandez Montechiesi, Breno Fernando Martins de Almeida

Creative Commons License

Este trabalho está licenciado sob uma licença Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

  • A Revista de Medicina Veterinária permite que o autor retenha os direitos de publicação sem restrições, utilizando para tal a licença  Creative Commons CC BY-NC-SA 4.0.
  • De acordo com os termos seguintes:
  • Atribuição — Você deve dar o crédito apropriado, prover um link para a licença e indicar se mudanças foram feitas. Você deve fazê-lo em qualquer circunstância razoável, mas de nenhuma maneira que sugira que o licenciante apoia você ou o seu uso.
  • NãoComercial — Você não pode usar o material para fins comerciais.
  • CompartilhaIgual — Se você remixar, transformar, ou criar a partir do material, tem de distribuir as suas contribuições sob a mesma licença que o original.
  • Sem restrições adicionais — Você não pode aplicar termos jurídicos ou medidas de caráter tecnológico que restrinjam legalmente outros de fazerem algo que a licença permita.