Supplementation of in vitro maturation medium with serum from dairy cows at early and late lactation in the in vitro production of bovine embryos

Authors

  • Letícia Franco Collares Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.
  • Jorgea Pradieé Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.
  • Morgana Alves Borges Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.
  • Bruna Mion Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.
  • Patrícia Gindri Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.
  • Janaína Fadrique Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.
  • Joao Alveiro Alvarado Rincón Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.
  • Lígia Margareth Cantarelli Pegoraro Empresa Brasileira de Pesquisa Agropecuária, Embrapa Clima Temperado, Pelotas-RS, Brasil.
  • Augusto Schneider Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.
  • Marcio Nunes Corrêa Programa de Pós-graduação em Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas-RS, Brasil.

DOI:

https://doi.org/10.26605/medvet-v12n4-2462

Keywords:

metabolism, oocyte maturation, transition period.

Abstract

The transition period of dairy cows is characterized by several metabolic alterations, due to the negative energy balance, which negatively affects fertility. The aim of this study was to evaluate the effect of supplementing the in vitro maturation medium with serum from dairy cows at early and late lactation in the oocyte maturation on the rates of nuclear maturation, cleavage and initial embryo development. The cumulus-oocyte complexes (COCs) were randomly divided into treatments: LL: serum from four cows at 354 ± 3.8 days in milk (DIM); and EL: serum from four cows at 10.8 ± 4.8 DIM. Subsequently, the COCs were submitted to maturation, fertilization; and in vitro culture in an incubator at 39°C with 5% CO2. For analysis of nuclear maturation; oocytes were collected after IVM, denuded and stained with Hoechst®. No difference was observed in the number of oocytes that reached metaphase II among treatments. The cleavage rate was not different between LL (59.3%; 320/540) and EL (47.0%; 243/517) treatments (P>0.05), as embryonic development between LL (15.0%; 81/540) and EL (16.6%; 86/517) (P>0.05). It was concluded that supplementation with serum from cows at different lactation periods during the bovine oocyte IVM did not influence the results of in vitro embryo production.

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References

Esposito, G.; Irons, P.C.; Webb, E.C.; Chapwanya, A. Interactions between negative energy balance, metabolic diseases, uterine health and immune response in transition dairy cows. Animal Reproduction Science, 144(3-4):6071, 2014.

Jaspard, B.; Collet, X.; Barbaras, R.; Manent, J.; Vieu, C.; Parinaud, J.;[ Perret, B. Biochemical characterization of pre‐beta 1 high‐ density lipoprotein from human ovarian follicular fluid: evidence for the presence of a lipid core. Biochemistry, 35:1352–1357, 1996.

Jorritsma, R.; César, M.L.; Hermans, J.T.; Kruitwagen, C.L.; Vos, P.L.; Kruip, T.A. Effects of non-esterified fatty acids on bovine granulosa cells and developmental potential of oocytes in vitro. Animal Reproduction Science, 81(3-4):225-35, 2004.

Leroy, J.L.; Van Soom, A.; Opsomer, G.; Bols, P.E. The consequences of metabolic changes in high-yielding dairy cows on oocyte and embryo quality. Animal, 2(8):1120-7, 2008 (a).

Leroy, J.L.; Van Soom, A.; Opsomer, G.; Goovaerts, I.G.; Bols, P.E. Reduced fertility in high-yielding dairy cows: are the oocyte and embryo in danger? Part II. Mechanisms linking nutrition and reduced oocyte and embryo quality in high-yielding dairy cows. Reproduction in domestic animals, 43(5):623-32, 2008 (b).

Leroy, J.L.; Vanholder, T.; Opsomer, G.; Van Soom, A.; de Kruif, A. The in vitro development of bovine oocytes after maturation in glucose and beta-hydroxybutyrate concentrations associated with negative energy balance in dairy cows. Reproduction in domestic animals, 41(2):119-23, 2006. Martinez, N.; Risco, C.A.; Lima, F.S.; Bisinotto, R.S.; Greco, L.F.; Ribeiro, E.S.; Maunsell, F.; Galvão K.; Santos, J.E.P. Evaluation of peripartal calcium status, energetic profile, and neutrophil function in dairy cows at low or high risk of developing uterine disease. Journal of Dairy Science, 95:7158–7172, 2012.

Mulligan, F.J,. Doherty, M.L. Production diseases of the transition cow. Veterinary Journal, 176(1):3-9, 2008.

Mullins, C.R.; Mamedova, L.K.; Brouk, M.J., Moore, C.E.; Green, H.B.; Perfield, K.L.; Smith, J.F.; Harner, J.P.; Bradford, B.J. Effects of monensin on metabolic parameters, feeding behavior, and productivity of transition dairy cows. Journal of Dairy Science, 95(3):132336, 2012.

O'Doherty, A.M.; O'Gorman, A.; al Naib, A.; Brennan, L.; Daly, E.; Duffy, P.; Fair, T. Negative energy balance affects imprint stability in oocytes recovered from postpartum dairy cows. Genomics, 104(3):177-85, 2014. Rearte, R.; LeBlanc, S. J.; Corva, S. G.; de la Sota, R. L.; Lacau-Mengido, I. M.; Giuliodori, M. J. Effect of milk production on reproductive performance in dairy herds. Journal of Dairy Science, 101:1–10, 2018. Rheingantz, M.G.T.; Pegoraro, L.M.C.; Dellagostin, O.A.; Pimentel, A.M.; Bernardi, M.L.; Deschamps, J.C. The sex ratio of in vitro produced bovine embryos is affected by the method of sperm preparation. Animal Reproduction, 3(4):423-430, 2006.

Rincón, J.; Madeira, E.M.; Campos, F.T.; Mion, B.; Silva, J.F.; Absalón-Medina, V.A.; Butler, W.R.; Corrêa, M.N.; Pegoraro, L.; Schneider, A. Exogenous paraoxonase-1 during oocyte maturation improves bovine embryo development in vitro. Reproduction in domestic animals, 51(5):827-30, 2016.

Rincón J.A.A.; Pradieé J.; Remião M.H; Collares T.V.; Mion,B.; Gasperin, B.G.; Rovani, M.T; Corrêa, M.N.; Pegoraro, L.M.C.; Schneider, A. Effect of high‐density lipoprotein on oocyte maturation and bovine embryo development in vitro. Reproduction in domestic animal, 00:1–11, 2018. Raboisson D, Mounié M, Maigné E.Diseases, reproductive performance, and changes in milk production associated with subclinical ketosis in dairy cows: a meta-analysis and review. Journal of Dairy Science 97(12):7547-

, 2014.

Van Hoeck, V.; Sturmey, R.G.; Bermejo-Alvarez, P.; Rizos, D.; Gutierrez-Adan, A.; Leese, H.J.; Bols, P.E.J.; Leroy, J.L.M.R. Elevated nonesterified fatty acid concentrations during bovine oocyte maturation compromise early embryo physiology. PloSOne, 6(8):23183, 2011.

Woudenberg, A.B.; Gröllers-Mulderij, M.; Snel, C.; Jeurissen, N.; Stierum, R.; Wolterbeek, A. The bovine oocyte in vitro maturation model: a potential tool for reproductive toxicology screening. Reproductive Toxicology, 34(2):251-60, 2012.

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Published

2019-04-03

How to Cite

Collares, L. F., Pradieé, J., Borges, M. A., Mion, B., Gindri, P., Fadrique, J., Rincón, J. A. A., Pegoraro, L. M. C., Schneider, A., & Corrêa, M. N. (2019). Supplementation of in vitro maturation medium with serum from dairy cows at early and late lactation in the in vitro production of bovine embryos. Medicina Veterinária, 12(4), 311–315. https://doi.org/10.26605/medvet-v12n4-2462

Issue

Vol. 12 No. 4 (2018)

Section

Animal Reproduction

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