ANTIBODIES AGAINST Leptospira spp. IN DAIRY CATTLE IN A FAMILY AGRICULTURE SYSTEM

Authors

DOI:

https://doi.org/10.26605/medvet-v18n2-6404

Keywords:

Family farming, dairy cattle, leptospirosis, diagnosis

Abstract

Leptospirosis is an endemic zoonosis in Brazil responsible for numerous reproductive and economic losses to the dairy industry. Brazil ranks fifth in the world's production of cow's milk and around 23 million cows are milked every day. The northwest region of the state of São Paulo has a considerable number of family farming settlements, with dairy farming being predominant. This article reports a seroepidemiological study to detect agglutinins against Leptospira spp. in 1,004 dairy cattle kept on 64 family farms producing milk in the northwest region of São Paulo. Management and environmental data for situation diagnosis were obtained with a questionnaire. Antibodies against Leptospira spp. were investigated by the microscopic seroagglutination test (SAM) and milk and urine samples were obtained from seroreactive animals with titer ? 400. These samples were submitted to molecular tests. Of the 64 properties studied, 63 (98%) had seroreactive animals. Of the 1,004 animals, 523 (52%) were reactive. According to the molecular tests, the presence of Leptospira spp. in the urine and milk of the seroreactive animals was negative. Wetlands, presence of rodents, occurrence of abortion, presence of dogs, presence of cats and presence of horses were variables associated with the risk of infection by Leptospira spp. In addition to its importance in animal health, leptospirosis poses a risk to human health, a situation that indicates the need for improvement of sanitary conditions in rural properties.

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References

Bharti A.R. et al. Leptospirosis: a zoonotic disease of global importance. Lancet Infectious Diseases, 3(12): 757-771, 2003.

BRASIL. Ministério da Saúde. Doenças Infecciosas e Parasitárias: Guia de Bolso. 8a ed. Brasília: Secretaria de Vigilância em Saúde, 2010. 444p.

Buainain, A.M.; Souza Filho, HM. Procera: Impactos produtivos e capacidade de pagamento, Relatório Preparado para o MEPF. Convênio FAO/INCRA, Brasília, 1998.

Castro, V. et al. Soroprevalência da leptospirose em fêmeas bovinas em idade reprodutiva no Estado de São Paulo, Brasil. Arquivos do Instituto Biológico, 75(1): 3-11, 2008.

Clazer, M. et al. Toxoplasmosis, leptospirosis, and brucellosis seroepidemiology in veterinary medical students and their relation with unique health. Semina: Ciências Agrárias, 38(3): 1347-1360, 2017.

Cosate, M.R. et al. Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock. BMC Veterinary Research, 13(177): 1-12, 2017.

Dewes, C. et al. Isolamento de Leptospira em feto equino abortado. Brazilian Journal of Development, 6(8): 62347-62354, 2020.

Di Azevedo, M.I.N. et al. Characterization of leptospiral DNA in the follicular fluid of non-pregnant cows. The Veterinary Record, 188(9): e143, 2021.

Diz, F.A.; Conceição, G.M.S. Leptospirose humana no município de São Paulo, SP, Brasil: distribuição e tendência segundo fatores sociodemográficos, 2007–2016. Revista Brasileira de Epidemiologia, 24: 1-14, 2021.

Faine, S. Guidelines for the control of leptospirosis, World Health Organization, Geneva, 1982. 171 p.

Favero, M. et al. Leptospirose bovina - variantes sorológicas predominantes em colheitas efetuadas no período de 1984 a 1994 em rebanhos de 21 Estados do Brasil. Arquivos do Instituto Biológico, 68(2): 29-35, 2001.

Favero, J.F. et al. Bovine leptospirosis: Prevalence, associated risk factors for infection and their cause-effect relation. Microbial Pathogenesis, 107: 149-154, 2017.

Ferreira, L.D.S. et al. Review of the main techniques of diagnostic of leptospirosis. Brazilian Journal of Health Review, 4(4): 15230-15243, 2021.

Fornazari, F. et al. Leptospira reservoirs among wildlife in Brazil: Beyond rodents. Acta Tropica, 178: 205-212, 2018.

Garba, B. et al. Retrospective study of leptospirosis in Malaysia. EcoHealth, 14(2): 389-398, 2017.

Genovez, M.E. et al. Serological profile of a nelore herd presenting endemic leptospirosis and submitted to vaccination. Arquivos do Instituto Biológico, 71(4): 411-416, 2004.

Guimarães, M.C.; Côrtes, J.A.; Vasconcellos, S.A. Epidemiologia e controle de leptospirose em bovinos. Papel do portador e seu controle terapêutico. Comunicação Científica da Faculdade de Medicina Veterinária e Zootecnia, USP, 6/7: 21-34, 1982/1983.

IBGE. Instituto Brasileiro de Geografia e Estatística. Sistema do Instituto Brasileiro de Geografia e Estatística de Recuperação Automática — SIDRA. IBGE, 2024. Available at: <http://www.sidra.ibge.gov.br/bda/pecua/default.asp>. Acessed on: 16 jun. 2024.

Jain, L. et al. Seroprevalence of brucellosis in bovines of Chhattisgarh, India. Indian Journal of Animal Research, 53(2): 255-259, 2019.

Juliano, R.S. et al. Prevalência e aspectos epidemiológicos da leptospirose bovina em rebanho leiteiro da microrregião de Goiânia- GO. Ciência Rural, 30(5): 857-862, 2000.

Lage, A.P. et al. Serology for Leptospira sp. in cattle of the State of Paraíba, Brazil. Arquivos do Instituto Biológico, 74(3): 185-190, 2007.

Lucchesi, P.M. et al. Recommendations for the detection of Leptospira in urine by PCR. Revista da Sociedade Brasileira de Medicina Tropical, 37: 131-134, 2004.

Luna, H.S. et al. Diagnóstico das condições do manejo sanitário e da saúde de bovinos criados no assentamento Vinte de Março localizado no município de Três Lagoas-MS. Revista Saúde e Meio Ambiente, 10(1): 32-42, 2020.

Maiolino, S.R. et al. Sperm viability, serological, molecular, and modified seminal plasma agglutination tests in the diagnosis of Leptospira in the semen and serum of bovine bulls. Brazilian Journal of Microbiology, 52(4):2431-2438, 2021.

Manzini, S. et al. First isolation of Leptospira kirschneri serovar canicola and Leptospira interrogans serovar pyrogenes in urine samples from slaughtered cattle in midwest region of São Paulo state, Brazil. Archives of Veterinary Science, 26(1): 51-62, 2021.

Martins, M.H.M.; Spink, M.J.P. Human leptospirosis as a doubly neglected disease in Brazil. Ciência & Saúde Coletiva, 25(3): 919-928, 2020.

Mérien, F. et al. Polymerase chain reaction for detection of Leptospira spp. in clinical samples. Journal of Clinical Microbiology, 30(9): 2219-2224, 1992.

Miller, D.A. et al. Porcine leptospirosis in Iowa. Journal of Veterinary Diagnostic Investigation, 2(3): 171-175, 1990.

Oca, O.Z.M.; Sanchez-Melorada, H.M.; Guerrero, A.V.M. La rata en la epizootiologia de la leptospirosis en granjas porcinas. Técnica Pecuária en México, 52: 29-44, 1986.

Oliveira, F.C.S. et al. Fatores de risco para a leptospirose em fêmeas bovinas em idade reprodutiva no Estado da Bahia, Nordeste do Brasil. Pesquisa Veterinária Brasileira, 30(5): 398-402, 2010.

Oliveira, S.V.; Arsky, M.L.N.S.; Caldas, E.P. Reservatórios animais da leptospirose: Uma revisão bibliográfica. Saúde (Santa Maria), 39(1): 9¬20, 2013.

Pietrafesa, J.P.; Alves, A.I.; Pietrafesa, P.A. The Social Division of Labor in Rural Spaces in Brazil: Memory and History of the Expansion of Rural Social Movements and Disputes Over Hegemony. Fronteiras: Journal of Social, Technological and Environmental Science, 7(2): 202-224, 2018.

Pinheiro, F.P. et al. Infectious diseases along Brazil's Trans-Amazon highway: surveilaance and research. Bulletin of the Pan American Health Organization, 8: 111-122, 1974.

Ribeiro, S.C.A.; et al. Leptospirose no rebanho bovino da sub-região de Nhecolândia, Pantanal Matogrossense, Brasil. Veterinária Notícias, 5(1): 51-55, 1999.

Rahman, T. et al. Zoonotic Diseases: Etiology, Impact, and Control. Microorganisms, 8: 1405, 2020.

Richtzenhain, L.J. et al. A multiplex PCR for the detection of Brucella spp. and Leptospira spp. DNA from aborted bovine fetuses. Veterinary Microbiolgy, 87(2): 139-147, 2002.

Santa Rosa, C.A. Diagnóstico laboratorial das leptospiroses. Revista de Microbiologia, 1(2): 97-109, 1970.

Santa Rosa, C.A. et al. Leptospirosis in wildlife in Brazil: isolation of serovars Canicola, Pyrogenes and Grippotyphosa. International Journal of Zoonosis, 7: 40-43, 1980.

Sant'ana, A.L. et al. Estratégias de Produção e Comercialização dos Assentados da Região de Andradina, Estado de São Paulo. Informações Econômicas, 37(5): 29-41, 2007.

Silva, F.J. et al. Prevalência e fatores de risco de leptospirose bovina no Estado do Maranhão. Pesquisa Veterinária Brasileira, 32(4): 303-312, 2012.

Thompson, J.A. et al. Spatial hierarchical variances and age covariances for seroprevalence to Leptospira interrogans serovar hardjo, BoHV-1 and BVDV for cattle in the State of Paraíba, Brazil. Preventive Veterinary Medicine, 76: 290-301, 2006.

Vasconcellos, S.A. et al. Leptospirose bovina. Níveis de ocorrência e sorotipos predominantes em rebanhos dos Estados de Minas Gerais, São Paulo, Rio de Janeiro, Paraná, Rio Grande do Sul e Mato Grosso do Sul, período de janeiro a abril de 1996. Arquivos do Instituto Biológico, 64(2): 7-15, 1997.

Veloso, I.F. et al. A comparison of three DNA extractive procedures with Leptospira for polymerase chain reaction analysis. Memórias do Instituto Oswaldo Cruz, 95(3): 339-43, 2000.

Zacarias, F.G.S. et al. Isolation of Leptospira serovars Canicola and Copenhageni from cattle urine in the state of Paraná, Brazil. Brazilian Journal of Microbiology, 39(4): 484-488, 2008.

Published

2024-08-02

How to Cite

Curci, V. C. M., Magajevski, F. S., Moreli, F. C. G., Romaldini , A. H. de C. N., Costa, R. L. D. da, Oliveira, T. C. B. de, Cardoso , T. C., Manzini , S., Cavalheiro, M. E., Alexandrino, M., Bertozzo, T. V., Steinle, J. S., Gomes, A. X. F., Aires, I. N., Lucheis , S. B., & Girio, R. J. S. (2024). ANTIBODIES AGAINST Leptospira spp. IN DAIRY CATTLE IN A FAMILY AGRICULTURE SYSTEM. Medicina Veterinária, 18(2), 159–168. https://doi.org/10.26605/medvet-v18n2-6404

Issue

Section

Veterinary Preventive Medicine