Use of histograms to predict hepatic steatosis in obese bitches submitted to the diet and its correlation with ultrasound findings (B-mode and Color Doppler)

Authors

  • Tamiris Disselli Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga-SP, Brasil https://orcid.org/0000-0002-7975-8994
  • Sâmara Turbay Pires Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga-SP, Brasil https://orcid.org/0000-0003-2376-2529
  • Anna Carolina Mazeto Ercolin Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga-SP, Brasil https://orcid.org/0000-0002-5832-9015
  • Camila Silveira Stanquini Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga-SP, Brasil https://orcid.org/0000-0003-2183-0751
  • Amanda Moura Rocha de Andrade Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga-SP, Brasil https://orcid.org/0009-0009-9830-9076
  • Danielle Passarelli Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga-SP, Brasil https://orcid.org/0000-0002-3893-5333
  • Adriano Rogério Bruno Tech Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga-SP, Brasil https://orcid.org/0000-0003-2628-1915
  • César Gonçalves de Lima Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga-SP, Brasil https://orcid.org/0000-0002-7244-4845
  • Diego Rodrigues Gomes Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga-SP, Brasil https://orcid.org/0000-0002-2410-7123
  • Denise Jaques Ramos Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga-SP, Brasil https://orcid.org/0000-0001-8687-3651
  • Maria Cristina Ferrarini Nunes Soares Hage Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga-SP, Brasil https://orcid.org/0000-0002-9309-774X

DOI:

https://doi.org/10.26605/medvet-v18n4-6600

Keywords:

fatty liver disease, weight loss, dogs, obesity, ultrasound

Abstract

It is known that ultrasonography is an accessible and economically viable diagnostic tool, being considered of choice for liver evaluation. The aim of this study was to evaluate qualitatively (B-mode ultrasound) and quantitatively (grayscale histograms) the liver of 28 bitches with different body condition scores (BCS) for the presence of fatty infiltration. The right hepatic vein (RHV) was evaluated with B mode and color Doppler mode for its visibility and presence of blood flow, respectively. Our hypothesis was that weight loss would reduce liver fat and, consequently, cease its mechanical effect on the venous lumen. Therefore, the dogs were divided into three groups, according to their BCS: control (BCS 4 or 5), overweight (BCS 6 or 7) and obese (BCS 8 or 9), which were subjected to diet, liver ultrasound monitoring and serial measurements of alkaline phosphatase (ALP) and alanine aminotransferase (ALT). The diet was effective for the animal’s weight loss, but the weight loss did not demonstrate effects on RHV or liver enzymes. The use of histograms contributed to the detection of liver fat and its reduction throughout the animal's weight loss. For that reason, it can be considered a useful and easy-to-use tool, contributing to the early diagnosis and monitoring of steatosis in a non-invasive way.

Downloads

Download data is not yet available.

References

Assawarachan, S.N. et al. Evaluation of hepatobiliary ultrasound scores in healthy dogs and dogs with liver diseases. Veterinary World, 12(8): 1266, 2019.

Banzato, T. et al. Quantitative analysis of ultrasonographic images and cytology in relation to histopathology of canine and feline liver: an ex-vivo study. Research in Veterinary Science, 103: 164-169, 2015.

Belotta, A.F. et al. Sonographic evaluation of liver hemodynamic indices in overweight and obese dogs. Journal of Veterinary Internal Medicine, 32: 181-187, 2018.

Brown, S. et al. Guidelines for the identification, evaluation, and management of systemic hypertension in dogs and cats. Journal of Veterinary Internal Medicine, 21(3): 542-558, 2007.

Brunetto, M.A. et al. Correspondência entre obesidade e hiperlipidemia em cães. Ciência Rural, 41(2): 266-271, 2011.

Carvalho, C.F. et al. Association of Doppler wave pattern of hepatic veins and fatty liver disease degree. Journal of Liver, 4: 1-5, 2015.

Coulden, R.A. et al. Doppler ultrasound of the hepatic veins: normal appearances. Clinical Radiology, 45(4): 223-227, 1992.

Després, J.P.; Lemieux, I. Abdominal obesity and metabolic syndrome. Nature, 444(7121): 881-887, 2006.

Eifler, R.V. The role of ultrasonography in the measurement of subcutaneous and visceral fat and its correlation with hepatic steatosis. Radiologia Brasileira, 46(5): 273-278, 2013.

Elliot, D.A. Techniques to assess body composition in dogs and cats. Whaltam Focus, 16: 16-20, 2006.

Fernandes, D.A. et al. Sonographic features associated with morbidity of chronic clinical presentations of schistosomiasis mansoni using the protocol proposed by the World Health Organization. Radiologia Brasileira, 46: 1-6, 2013.

Gossellin, J. et al. Canine obesity: An overview. Journal of Veterinary Pharmacology Therapeutics, 30(1): 1-10, 2007.

Hu, H.H. et al. Comparison of fat-water MRI and single-voxel MRS in the assessment of hepatic and pancreatic fat fractions in humans. Obesity (Silver Spring), 18(4): 841-847, 2010.

Jericó, M.M. Obesidade e hiperlipidemias. Vets Today, 5, 2011. Disponível em: <http://conteudo.royalcanin.com.br/upload/InformativoVET%20TodayN52011(c).pdf>. Acesso em: 22 nov. 2023.

Laflamme, D.P. Development and validation of a body condition score system for dogs. Canine Practice, 22(1): 10-15, 1997.

Lee, K. et al. Comparison of ultrasound histogram of liver, kidney and spleen in beagle dogs. Journal of Veterinary Clinics, 22(3), 186-189, 2005.

Lessa, A.S. et al. Ultrasound imaging in an experimental model of fatty liver disease and cirrhosis in rats. BMC Veterinary Research, 6(1): 6, 2010.

Marchi, P.N. et al. Metabolic parameters in obese dogs undergoing to the diet with calorie restriction. Open Journal of Veterinary Medicine, 8(3): 25-35, 2018.

Mattoon, J.S. et al. Small Animal Diagnostic Ultrasound. 4th ed. St. Louis: Elsevier Health Sciences, 2020. 704p.

Pereira Neto, G.B. et al. Effects of weight loss on the cardiac parameters of obese dogs. Pesquisa Veterinária Brasileira, 30(2): 167-171, 2010.

Osto, M.; Lutz, T.A. Translational value of animal models of obesity-Focus on dogs and cats. European Journal of Pharmacology, 759: 240-252, 2015.

Paige, J.S. et al. A pilot comparative study of quantitative ultrasound, conventional ultrasound, and MRI for predicting histology-determined steatosis grade in adult nonalcoholic fatty liver disease. American Journal of Roentgenology, 208(5): W168-W177, 2017.

Rothuizen, J.; Twedt, D.C. Liver biopsy techniques. Veterinary Clinical North American Small Animal Practitioner, 39(3): 469-480, 2009.

Schwenzer, N.F. et al. Non-invasive assessment and quantification of liver steatosis by ultrasound, computed tomography and magnetic resonance. Journal of Hepatology, 51(3): 433-445, 2009.

Shepherd, M. Canine and feline obesity management. Veterinary Clinical North American Small Animal Practitioner, 51(3): 653-667, 2021.

Syakalima, M. et al. Comparison of attenuation and liver?kidney contrast of liver ultrasonographs with histology and biochemistry in dogs with experimentally induced steroid hepatopathy. Veterinary quarterly, 20(1): 18-22, 1998.

Smithenson, B.T. et al. Pulsed-wave Doppler ultrasonographic evaluation of hepatic veins during variable hemodynamic states in healthy anesthetized dogs. American Journal of Veterinary Research, 65(6): 734-740, 2004.

Webb, M. et al. Diagnostic value of a computerized hepatorenal index for sonographic quantification of liver steatosis. American Journal of Roentgenology, 192(4): 909-914, 2009.

Zoran, D.L. Obesity in dogs and cats: A metabolic and endocrine disorder. Veterinary Clinics of North America: Small Animal Practice, 40(2): 221-239, 2010.

Published

2024-12-18

How to Cite

Disselli, T., Pires, S. T., Ercolin, A. C. M., Stanquini, C. S., Andrade, A. M. R. de, Passarelli, D., Tech , A. R. B., Lima, C. G. de, Gomes, D. R., Ramos, D. J., & Hage, M. C. F. N. S. (2024). Use of histograms to predict hepatic steatosis in obese bitches submitted to the diet and its correlation with ultrasound findings (B-mode and Color Doppler). Medicina Veterinária, 18(4), 331–339. https://doi.org/10.26605/medvet-v18n4-6600

Issue

Section

Small Animal Clinic and Surgery

Most read articles by the same author(s)