Pre or perioperative period: when is the best time to produce platelet-rich plasma in dogs?
(Pré ou trans-operatório: qual o melhor momento para a produção do plasma rico em plaquetas em cães?)
DOI:
https://doi.org/10.26605/medvet-v16n3-4984Keywords:
surgery, platelet concentrate, growth factorsAbstract
When evaluating published studies on the production of platelet-rich plasma (PRP), it is possible to observe that there is no consensus on when blood should be collected and processed. Some perform blood collection before surgery, prior to the administration of drugs, fluids, or any surgical trauma, while others collect blood during the perioperative period. The present objective was to determine the best moment for PRP production based on the platelet count. Blood samples were collected from 20 healthy female dogs at two distinct moments: before surgery, prior to anesthesia and fluid therapy (M0), and later, in the operation room, after administration of the anesthetics and intravenous fluids (M1). To obtain PRP, blood was centrifuged at 1200 rpm for ten minutes, then at 1600 rpm for ten minutes. Results showed that the blood collected at M1 had a 29% decrease in platelet count compared to M0 (highly significant statistical difference). It is recommended that blood collection, which is an essential step for producing platelet-rich plasma, shall be done during the pre-operative period.Downloads
References
Adler, S.C.; Kent, K.J. Enhancing wound healing with growth factors. Facial Plastic Surgery Clinics of North America, 10: 129-146, 2002.
Al-Dirawi, A. et al. Clinical and radiological evaluation of transplanted fresh ear cartilage impregnated with the mesenchymal cells & prp in treatment of growth plate injury in lamb. Iranian Journal of Veterinary Medicine, 12(4): 283-291, 2018.
Alves, R.; Grimalt, R.A. Review of Platelet-Rich Plasma: History, Biology, Mechanism of Action, and Classification. Skin Appendage Disorders, 4(1): 18?24, 2018.
Andrade, S.F. Fluidoterapia e transfusão sanguínea. In:____. Manual de Terapêutica Veterinária. São Paulo: Roca, 2018. p. 562-579.
Barbosa, A.L.T. et al. Plasma rico em plaquetas para reparação de falhas ósseas em cães. Ciência Rural, 38(5): 1335-1340, 2008.
Barroso, C.S.T. et al. Calidad del plasma rico en plaquetas: estúdio de la activación plaquetaria. Revista Española de Cirugía Oral y Maxilofacial, 29(4): 240-248, 2007.
Boyd, C.J. et al. Hypocoagulability and platelet dysfunction are exacerbated by synthetic colloids in a canine hemorrhagic shock model. Frontiers in Veterinary Science, 5: 279, 2018.
Carr, B.J. et al. Canine platelet-rich plasma systems: a prospective analysis. Frontiers in Veterinary Science, 2: 73, 2016.
Chohan, A.S. et al. Effects of 6% hetastarch (600/0.75) or lactated ringer's solution on hemostatic variables and clinical bleeding in healthy dogs anesthetized for orthopedic surgery. Veterinary Anaesthesia and Analgesia, 38(2): 94-105, 2011.
Conceição, M.L. et al. Comparative study of double spin and use of e-pet filter (equine platelet enhancement therapy) to obtain platelet rich plasma in horses. Pesquisa Veterinária Brasileira, 37(3): 215-220, 2017.
Conner, B.J. et al. Effects of acepromazine maleate on platelet function assessed by use of adenosine diphosphate activated- and arachidonic acid- activated modified thromboelastography in healthy dogs. American Journal of Veterinary Research, 73(5): 595-601, 2012.
Dalgin, D. et al. Platelet rich plasma (prp) treatment in a dog with heavily injured tail due to tail chasing behavior. Harran University Journal of the Faculty of Veterinary Medicine, 6(1): 99-101, 2017.
Elyazji, N.R. et al. The effects of tramadol hydrochloride administration on the hematological and biochemical profiles of domestic male rabbits. IUG Journal of Natural and Engineering Studies, 21(2): 51-65, 2013.
Foster, T.E. et al. Platelet-rich plasma: from basic science to clinical applications. The American Journal of Sports Medicine, 37(11): 2259-2272, 2009.
Franklin, S.P. et al. Characteristics of canine platelet-rich plasma prepared with five commercially available systems. American Journal of Veterinary Research, 76(9): 822–827, 2015.
Fry, M.M. Acquired platelet dysfunction. In: Weis, D.J.; Wardrop, K.J. Schalm’s Veterinary Hematology. Oxford: Blackwell Publishing, 2010. p.626-630.
Garcez, T.N.A. et al. Plasma rico em plaquetas e suas frações líquidas e em gel como adjuvantes da cicatrização cutânea. Acta Scientiae Veterinariae, 44(1355): 1-7, 2016.
Hauschild, G.F. et al. Short term storage stability at room temperature of two different platelet-rich plasma preparations from equine donors and potential impact on growth factor concentrations. BMC Veterinary Research, 13(1): 7, 2017.
Imam, S.S. Role of platelet rich plasma mediated repair and regeneration of cell in early stage of cardiac injury. Regenerative Therapy, 19: 144-153, 2022.
Kemper, B. et al. Autologous and homologous skin grafts treated with platelet-rich plasma (PRP): experimental study in rabbits. Pesquisa Veterinária Brasileira, 38(9): 1818-1823, 2018.
Landi, E.P.; Marques Júnior, J.F.C. Caracterização da ativação plaquetária nos concentrados de plaquetas por citometria de fluxo. Revista Brasileira de Hematologia e Hemoterapia, 25(1): 39-46, 2003.
Miranda, S. et al. Protocols for preparation of platelet rich plasma (PRP) in Quarter Horses. Pesquisa Veterinária Brasileira, 39(8): 614-621, 2019.
O'rourke, L.G. Preclinical evaluation of compound-related cytopenias. In: Weis, D.J.; Wardrop, K.J. Schalm’s Veterinary Hematology. Oxford: Blackwell, 2010. p.85-91.
Ozgul, U. et al. Effects of isoflurane and propofol on hepatic and renal functions and coagulation profile after right hepatectomy in living donors. Transplantation Proceedings, 45(3): 966-970, 2013.
Pazzini, J.M. et al. Emprego de plasma rico em plaquetas associado à esponja cirúrgica em enxertos cutâneos em coelhos (Oryctolagus cuniculus). Revista Brasileira de Medicina Veterinaria, 38: 397-405, 2016.
Sethi, D. et al. Systematic literature review evaluating evidence and mechanisms of action for platelet-rich plasma as an antibacterial agent. Journal of Cardiothoracic Surgery. 16: 277, 2021.
Silva, G.C. Plasma Rico em Plaquetas (PRP) autólogo: Evidências dos benefícios na cicatrização de úlceras. Ânima Runa, 17: 17-18, 2021.
Singh, R. et al. To evaluate the role of platelet-rich plasma in healing of acute diaphyseal fractures of the femur. Chinese Journal of Traumatology, 20(1): 39-44, 2017.
Stockham S.L.; Scott, M. A. Fundamentos de Patologia Clínica Veterinária. 2nd ed. Rio de Janeiro: Guanabara Koogan, 2011. 729 p.
Suaid, F.F. et al. Avaliação clínica da associação do plasma rico em plaquetas (PRP) com o enxerto de tecido conjuntivo subepitelial em retrações gengivais criadas em cães. Periodontia, 17(1): 68-75, 2007.
Sutil, D.V. et al. Hematological and splenic doppler ultrasonographic changes in dogs sedated with acepromazine or xylazine. Veterinary Anesthesia and Analgesia, 44(4): 746-754, 2017.
Tavares, D.C. et al. Splenic congestion associated with acepromazine administration in dogs. Brazilian Journal of Veterinary Research and Animal Science, 51(4): 304-308, 2014.
Thepsoparn, M. Comparison of a Platelet-Rich Plasma Injection and a Conventional Steroid Injection for Pain Relief and Functional Improvement of Partial Supraspinatus Tears. Orthopaedic Journal of Sports Medicine, 9(9): 7, 2021.
Thrall, M.A. et al. Hematologia e Bioquímica Clínica Veterinária. São Paulo: Roca, 2015. p. 86-99.
Vendramin, F.S. et al. Utilização do plasma rico em plaquetas (PRP) autólogo em enxertos cutâneos em coelhos. Revista Brasileira de Cirurgia Plástica, 25(1): 4-10, 2010.
Zimmerman, K.L. Drug-induced thrombocytopenias. In: Feldman, B.F. et al. Schalm’s Veterinary Hematology. 5th ed. ed. Philadelphia: Lippincott Williams & Wilkins, 2000. p. 472-477
Weiser, G. Coleta e processamento da amostra e análise das opções de serviço laboratorial. In: Thrall, M.A. et al. Hematologia e Bioquímica Clínica Veterinária. São Paulo: Roca, 2015. p. 86-99.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
A Revista de Medicina Veterinária permite que o autor retenha os direitos de publicação sem restrições, utilizando para tal a licença Creative Commons CC BY-NC-SA 4.0.
De acordo com os termos seguintes:
Atribuição — Você deve dar o crédito apropriado, prover um link para a licença e indicar se mudanças foram feitas. Você deve fazê-lo em qualquer circunstância razoável, mas de nenhuma maneira que sugira que o licenciante apoia você ou o seu uso.
NãoComercial — Você não pode usar o material para fins comerciais.
CompartilhaIgual — Se você remixar, transformar, ou criar a partir do material, tem de distribuir as suas contribuições sob a mesma licença que o original.
Sem restrições adicionais — Você não pode aplicar termos jurídicos ou medidas de caráter tecnológico que restrinjam legalmente outros de fazerem algo que a licença permita.
