Trans-ferulic acid on the in vitro maturation of ovine zygotes
DOI:
https://doi.org/10.26605/medvet-v18n3-6502Keywords:
antioxidant, in vitro fertilization, in vitro maturation, sheepAbstract
This work aimed to evaluate the use of trans-ferulic acid (ATF) in the in vitro maturation of oocytes and in the in vitro production of ovine embryos. For this, oocytes were divided into five treatment groups: the control (CON) group, in which oocytes were immersed in medium without the use of antioxidants, while in the cysteamine (CIS) group, the oocytes were included in the same medium as the CON group, but containing 100µM of cysteamine as an antioxidant source; in the ATF10, ATF50, ATF100 groups, the oocytes were included in the same medium as in the CON group, but containing 10µM, 50µM and 100µM trans-ferulic acid, respectively. In vitro maturation (IVM) was performed for 24h, at 38.5°C, with 5% of carbon dioxide (CO2). Then, in vitro fertilization (IVF) was performed, followed by in vitro culture (IVC) of the presumed zygotes, which occurred in synthetic oviductal fluid (SOF) medium with 2mg/mL of bovine serum albumin (BSA), evaluating the rate of cleaved structures after 48h of cultivation. Data were presented in percentages and the Chi-square test was used in the Epi Info® software. The results were considered significant when p?0.05. It was possible to observe that about the expansion rate, AFT100 was superior to ATF10, CIS, and CON (95.3%; 76.7%; 79.1%; 81.4%). For the expansion degree of cumulus cells, the number of presumed zygotes, and the number of cleaved structures, there was no significant difference between the groups with trans-ferulic acid (ATF10, ATF50, and ATF100) and cysteamine (CIS). It is possible to conclude that trans-ferulic acid is an alternative to cysteamine as an antioxidant agent in the in vitro maturation medium of ovine oocytes.Downloads
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Copyright (c) 2024 Andreza Mayara Carneiro de Lima, Luana Kealy Pimentel de Oliveira, Érika Karoline de Oliveira Aureliano, Damaris Raquel Pires dos Santos, Beatriz Cavalcanti de Freitas, William Matheus Souza, Jossimara de Melo Silva, Ailton Batista Pereira, Sueli de Oliveira Lima, Isabela Maria Lopes, Bruna Moura Silva, Thaís Thatiane dos Santos Souza, Mabel Freitas Cordeiro, Edilson Soares Lopes Júnior
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