Evaluation of the potential of the fungus Pochonia chlamydosporia (VC4) in the biological control of Alphitobius diaperinus under laboratory conditions
DOI:
https://doi.org/10.26605/medvet-v18n3-6846Keywords:
poultry farming, lesser mealworm, nematophagous fungi, pest controlAbstract
Alphitobius diaperinus, commonly known as lesser mealworm or darkling beetle, is currently one of the main problems in global poultry farming. The aim of this study was to evaluate the potential of the fungus Pochonia chlamydosporia (VC4) in the biological control of A. diaperinus under laboratory conditions. Adult specimens of the insect were collected from a poultry farm in southeast Brazil. Subsequently, the fungus P. chlamydosporia was grown on chitin agar medium (2% AQ). After 10 days, a concentration of fungal structures (conidia/chlamydospores) of 1500/?L was obtained. Later, two groups were formed: group (G1), consisting of Petri dishes containing 2% water agar culture medium (AA2%) with 10 live insects + 10µL of fungal solution (poured) over each insect; and the control group (G2), which consisted of Petri dishes containing 2% AA + 10 live insects + 10µL of distilled water poured over each insect. Both groups were conducted in triplicate. The plates of both groups G1 and G2 were incubated in a BOD at 25±1°C and 80±10% relative humidity for 7 days. At the end of the seventh day of interaction, in group G1, colonization and death of 100% of A. diaperinus were observed, along with the visualization of VC4 hyphae on them, suggesting a possible entomopathogenic activity. In group G2, live insects were observed. It is concluded that the biological control of A. diaperinus by the fungus P. chlamydosporia (VC4) was effective under laboratory conditions, and further experimental designs under field conditions may contribute to its applicability.Downloads
References
ABPA. Associação Brasileira de Proteína Animal. Dados gerais da avicultura e suinocultura em 2022. Relatório Anual 2023. Disponível em: <https://abpa-br.org/wp-content/uploads/2023/04/Relatorio-Anual-2023.pdf>. Acesso em: 1º mar. 2024.
Alves, L.F.A.; Alvez, V.S.; Bressan, D.F.; Pedro, M.O.J.; Neves, P.M.O.J.; Alves, S.B. Ocorrência de Metarhizium anisopliae (Metsch.) Sorok. (Moniliales: Moniliaceae) em adultos de cascudinho (Alphitobius diaperinus) (Panzer) (Coleoptera: Tenebrionidae), em aviários comerciais em Cascavel, PR. Neotropical Entomology, 33: 793-795, 2004.
Braga, F.R.; Araújo, J.V.; Soares, F.E.F.; Araujo, J.M.; Tavela, A.O.; Carvalho, L.M.; Mello, I.N.K.; de Paula, A.T.; Lelis, R.; Queiroz, J.H. Interaction of the nematophagous fungus Duddingtonia flagrans on Amblyomma cajannense engorged females and enzymatic characterisation of its chitinase. Biocontrol Science and Technology, 23(5): 584-594, 2013.
Braga, F.R.; de Araújo, J.V. Nematophagous fungi for biological control of gastrointestinal nematodes in domestic animals. Applied Microbiology and Biotechnology, 98(1): 71-82, 2014.
Chernaki-leffer, A.M.; Almeida, L.M. Exigências térmicas, período de desenvolvimento e sobrevivência de imaturos Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). Neotropical Entomology, 30: 365-368, 2001.
Cohen, E. Chitin synthesis and degradation as targets for pesticide action. Archives of Insect Biochemistry Physiology, 22: 245–261, 1993.
Crippen, T.L.; Sheffield, C.L.; Beier, R.C.; Nisbet, D.J. The horizontal transfer of Salmonella between the lesser mealworm (Alphitobius diaperinus) and poultry manure. Zoonosis and Public Health, 65: 23-33, 2018.
Daniel, J.F.S.; Scalco, A.V.; Souza, R.M.; Ocampos, F.M.M.; Barison, A.; Alves, L.F.A.; Neves, P.M.O.J. Susceptibly of Alphitobius diaperinus to Beauveria bassiana extracts. Natural Product Research, 33: 3033-3036, 2019.
Dinev, I. The darkling beetle (Alphitobius diaperinus) – a health hazard for broiler chicken production. Trakia Journal of Science, 1: 1-4, 2013.
Esteves, I.; Peteira, B.; Atkins, S.D.; Magan, N.; Kerry, B. Production of extracellular enzymes by different isolates of Pochonia chlamydosporia. Mycological Research. 113(8): 867-76, 2009.
Fabritius, H.; Sachs, C.; Raabe, D.; Nikolov, S.; Friák, M.; Neugebauer, J. Chitin in the exoskeletons of Arthropoda: From Ancient Design to Novel Materials Science. In: Gupta, N.S. Chitin: topics in geobiology. Dordrecht: Springer, 2011. p.35-60.
Ferraz, C.M.; Soares, F.E.F.; Sena, C.C.; Silva, L.P.C.; Araújo, J.V.; Moreira, T.F.; Monteiro, F.C.; Braga, F.B. Interaction of the nematophagous fungus Pochonia chlamydosporia on eggs of Spartocera dentiventris (Berg) (Hemiptera: Coreidae) under laboratory conditions. Brazilian Journal of Biology, 81(4): 1122-1124, 2021.
Fonseca, J.S.; Altoé, L.S.C.; Carvalho, L.M.; Soares, F.E.F.; Braga, F.R.; Araújo, J.V. Nematophagous fungus Pochonia chlamydosporia to control parasitic diseases in animals. Applied Microbiology and Biotechnology, 107(12): 3859-3868, 2023.
García, L.; Bulnes, C.; Melchor, G.; Vega, E.; Ileana, M.; de Oca, N.M.; Hidalgo, L.; Marrero, E. Safety of Pochonia chlamydosporia var catenulata in acute oral and dermal toxicity/pathogenicity evaluations in rats and rabbits. Veterinary and Human Toxicology, 46(5): 248-250, 2004.
Gehring, V.S.; Santos, E.D.; Mendonça, B.S.; Santos, L.R.; Rodrigues, L.B.; Dickel, E.L.; et al. Alphitobius diaperinus control and physicochemical study of poultry litters treated with quicklime and shallow fermentation. Poultry Science, 99: 2120-2124, 2020.
Rohde, C.; Alves, L.F.A.; Neves, P.M.O.J.; Alves, S.B.; da Silva, E.R.L.; de Almeida, J.E.M. Selection of Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok. isolates against Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). Neotropical Entomology, 35(2): 231-240, 2006.
Schmidt, N.S.; Silva, C.L. Pesquisa e Desenvolvimento na Cadeia Produtiva de Frangos de Corte no Brasil. Revista de Economia e Sociologia Rural, 56(3): 467-482, 2018.
Senna, C.C.; Sena, F.P.; da Paz, J.S.; de Barros Rios, A.; Ferraz, C.M.; Lenz, D.; Soares, F.E.F.; Tobias, F.L.; Hiura, E.; de Araújo, J.V.; Braga, F.R. Colonization and destruction of ants of the genus Camponotus sp. (Hymenoptera: Formicidae) in vitro by the fungus Pochonia chlamydosporia in the southeast region of Brazil. 3 Biotech, 8(8): 333, 2018.
Singh, N.; Johnson, D. Baseline Susceptibility and Cross-Resistance in Adult and Larval Alphitobius diaperinus (Coleoptera: Tenebrionidae) Collected from Poultry Farms in Arkansas. Journal of Economic Entomology, 108: 1994–1999, 2015.
Smith, R.; Hauck, R.; Macklin, K.; Price, S.; Dormitorio, T.; Wang, C. A review of the lesser mealworm beetle (Alphitobius diaperinus) as a reservoir for poultry bacterial pathogens and antimicrobial resistance. World's Poultry Science Journal, 78(1): 197-214, 2022.
Valadão, M.C.; Carvalho, L.M.; Vieira, I.S.; Neves, P.H.; Ferreira, V.M.; Campos, A.K.; Soares, F.E.F.; Ferraz, C.M.; Vilela, V.L.R.; Braga, F.R.; Araújo, J.V. Germination capacity of the Pochonia chlamydosporia fungus after its passage through the gastrointestinal tract of domestic chickens (Gallus gallus domesticus). Experimental Parasitology, 216: 107936-107940, 2020.
Wolf, J.; Gouvea, A.; Silva, E.R.L.; Potrich, M.; Appel, A. Physical methods and hydrated lime for management of lesser mealworm. Ciência Rural, 44(1): 161-166, 2014.
Wolf, J.; Potrich, M.; Lozano, E.R.; Gouvea, A.; Pegorini, C.S. Combined physical and chemical methods to control lesser mealworm beetles under laboratory conditions. Poultry Science, 94(6): 1145-1149, 2015.
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Copyright (c) 2024 João Pedro Barbosa Assis, Carolina Magri Ferraz, Luciara Paiva, Debora Castro Souza, Filippe Elias Freitas Soares, Fernando Luiz Tobias, Thaís Schmidt Ferreira, Jackson Victor Araújo, Vinícius Longo Ribeiro Vilela, Fabio Ribeiro Braga
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