Characterization and in vitro susceptibility profile of bacterial samples harvest from canine chronic otitis
DOI:
https://doi.org/10.26605/medvet-v15n2-4396Palabras clave:
aminoglycosides, antibiogram, ototoxicity, quinolones, Staphylococcus spp.Resumen
This study aimed to identify which are the most frequent bacteria evolved in cases of chronic otitis in dogs in the metropolitan region of Curitiba, as well to determine their in vitro antimicrobial susceptibility. Data of positive bacterial culture from dogs affected by chronic or recurrent otitis were compiled from the records of the veterinary hospital of Pontifícia Universidade Católica do Paraná, Curitiba, southern Brazil. In a period of 16 months, a total of 83 bacterial cultures were performed, resulting in 192 isolates. All isolates were submitted to antimicrobial susceptibility tests, based on the Kirby-Bauer technique using 17 drugs from 8 antibiotic classes (?-lactams, aminoglycosides, lincosamides, macrolides, polypeptides, quinolones, tetracyclines, and amphenicols). The five most frequent bacterial isolates were Staphylococcus spp. (58.32%), Proteus spp. (14.58%), Escherichia coli (9.90%) and Pseudomonas spp. (8.33%). The four most effective antibiotics were amikacin (13.29%), neomycin (24.47%), gentamicin (25.52%) and tobramycin (26.70%); however, these aminoglycosides may cause ototoxicity, and their use should be restricted when the tympanic membrane is intact. Quinolones also showed antimicrobial effectiveness, with 29.17% of the isolates showing resistance to ciprofloxacin and 29.69% to enrofloxacin. According to the results, it can be concluded that aminoglycosides and quinolones were effective against microorganisms of canine chronic otitis.Descargas
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Angus, J.C. Pathogenesis of otite externa: Understanding primary causes. Proceeding of the North American Veterinary Conference, Orlando, Florida, pp. 807-809, 2005.
Bauer, A.W.; Kirby, W.M.; Sherris, J.C.; Turck, M. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45: 493-496, 1966.
Bensignor, E. Aproximación a la otitis externa y a la otitis media. In: Foster, A.; Foils, C. (eds). Manual de dermatología en pequeños animales y exóticos. 2nd ed. L’Hospitalet de Llobregat: Ediciones S., 2008. p. 143-153.
Bugden, D.L. Identification and antibiotic susceptibility of bacterial isolates from dogs with otitis externa in Australia. Australian Veterinary Journal, 91(1-2): 43-46, 2013.
Cheng, A.; Chuang, Y.C.; Sun, H.Y.; Sheng, W.H.; Yang, C.J.; Liao, C.H.; Hsueh, P.R.; Yang, J.L.; Shen, N.J.; Wang, J.T.; Hung, C.C.; Chen, Y.C.; Chang, S.C. Excess mortality associated with colistin-tigecycline compared with colistin-carbapenem combination therapy for extensively drug-resistant Acinetobacter baumannii bacteremia: A multicenter prospective observational study. Critical Care Medicine, 43(6): 1194-1204, 2015.
Cole, L.K. Anatomy and physiology of the canine ear. Veterinary Dermatology, 20: 412-421, 2009.
Cole, L.K. Otitis externa. In: Greene, C.E. Infectious diseases of the dog and cat. 4th ed. St Louis: Elsevier Saunders, 2012. p. 885-892.
Colombini, S.O. Dermatología: enfermedades pruríticas de la piel en perros y gatos. 1st ed. Delaware: The Gloyd Group, 2005. 68 p.
Daniel, S.J.; Munguia, R. Ototoxicity of topical ciprofloxacin/dexamethasone otic suspension in a chinchilla animal model. Otolarygology-Head and Neck Surgery, 139: 840-845, 2008.
Giguère, S.; Prescott, J.F.; Dowling, P.M. Antimicrobial therapy in veterinary medicine. Iowa: Wiley Blackwell, 2013.
Girão, M.D.; Prado, M.R.; Brilhante, R.S.N.; Cordeiro, R.A.; Monteiro, A.J.; Sidrim, J.J.C.; Rocha, M.F.G. Malassezia pachydermatis isolated from normal and diseased external ear canals in dogs: A comparative analysis. The Veterinary Journal, 172: 544-548, 2006.
Gotthelf, G.N. Doenças do ouvido em pequenos animais. 2ª ed. São Paulo: Editora Roca, 2007. 356p.
Griffin, C.E. Diagnosis of otitis externa and media – The PSPP approach. Proceedings of the 27th Annual Congress of the Esvid-Ecvd; 2014a; Set 11-13; Salzburg, Austria. p. 24-26.
Griffin, C.E. Medical treatment of otitis. Proceedings of the 27th Annual Congress of the Esvid-Ecvd; 2014b; Set 11-13; Salzburg, Austria. p. 32-34.
Hariharan, H.; Coles, M.; Poole, D.; Lund, L.; Page, R. Update on antimicrobial susceptibilities of bacterial isolates from canine and feline otitis externa. Canadian Veterinary Journal, 47: 253-255, 2006.
Harvey, R.G.; Harari, J.; Delauche, A.J. Doenc?a do ouvido em ca?es e gatos. Rio de Janeiro: Revinter, 2004. 272 p.
Huang, J.T.; Abrams, M.; Tlougan, B.; Rademaker, A.; Paller, A.S. Treatment of Staphylococcus aureus colonization in atopic dermatitis decreases disease severity. Pediatrics, 123: e808-e814, 2009.
Jacobson, L.S. Diagnosis and medical treatment of otitis externa in the dog and cat. Journal of the South African Veterinary Association, 73(4): 162-170, 2002.
Lemke, L.E.; McGee, D.H.; Prieskorn, D.M.; Wall, G.M.; Dolan, D.F.; Altschuler, R.A.; Miller, J.M. Safety of ciprofloxacin and dexamethasone in the guinea pig middle ear. Archives of Otolaryngology-Head & Neck Surgery, 135(6): 575-580, 2009.
Luz, G.P.; Anater, A.; Ribeiro, D.R.; Fraiz, F.V.; Bárcena, J.; Kung, D.C.; Farias, M.R.; Pimpão, C.T. Avaliação da eficácia da associação de tiabendazol, sulfato de neomicina, dexametasona e cloridrato de lidocaína no tratamento da otoacaríase. Ciências Agrárias e Ambientais (Online), 12(4): 260-269, 2014.
Magiorakos, A.P.; Srinivasan, A.; Carey, R.B.; Carmeli, Y.; Falagas, M.E.; Giske, C.G.; Harbarth, S.; Hindler, J.F.; Kahlmeter, G.; Olsson-Liljequist, B.; Paterson, D.L.; Rice, L.B.; Stelling, J.; Struelens, M.J.; Vatopoulos, A.; Weber, J.T.; Monnet, D.L. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance. Clinical Microbiology and Infection, 18(3): 268-281, 2012.
Malayeri, H.Z.; Jamshidi, S.; Salehi, T.Z. Identification and antimicrobial susceptibility patterns of bacteria causing otitis externa in dogs. Veterinary Research Communications, 34: 435-444, 2010.
Marinho, P.V.T.; Nunes, G.D.L.; Filgueira, F.G.F.; Tavares, T.H.; Silva, C.M.A.; Leite, A.R.A.; Carneiro, R.S.; Garino Júnior, F. Multirresistência bacteriana em cães com otite bilateral crônica recidivante (Relato de caso). Anais da IX Jornada de Ensino, Pesquisa e Extensão, 2009; Out 19-23; Pernambuco, Brasil. p. 1-3.
Matousek, J.L. Diseases of the ear pinna. Veterinary Clinic of North American Small Animal Practice, 34(2): 511-540, 2004.
Miller, W.; Griffin, C.; Campbell, K. Muller and Kirk's small animal dermatology. 7th ed. Philadelphia: WB Saunders, 2013.
Moraes, L.F.; Takahira, R.K. Aplasia medular em cães. Revista de Ciências Agroveterinárias, 9(1): 99-108, 2010.
Moreira, C.A.; Oliveira, L.C.; Mendes, M.S.; Santiago, T.M.; Barros, E.B.; Carvalho, C.B.M. Biofilm production by clinical Staphylococci strains from canine otitis. Brazilian Journal of Microbiology, 43(1): 371-374, 2012.
Morris, D.O. Medical therapy of otitis externa and otitis media. Veterinary Clinics of North American Small Animal Practice, 34(2): 541-555, 2004.
Oliveira, L.C.; Medeiros, C.M.O.; Silva, I.N.G.; Monteiro, A.J.; Leite, C.A.L.; Carvalho, C.B.M. Susceptibilidade a antimicrobianos de bactérias isoladas de otite externa em cães. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 57(3): 405-408, 2005.
Oliveira, L.C.; Leite, C.A.L.; Brilhante, R.S.N.; Carvalho, C.B.M. Comparative study of the microbial profile from bilateral canine otitis externa. Canadian Veterinary Journal, 49: 785-788, 2008.
Penna, B.; Varges, R.; Medeiros, L.; Martins, G.M.; Martins, R.R.; Lilenbaum, W. Species distribution and antimicrobial susceptibility of Staphylococci isolated from canine otitis externa. Veterinary Dermatology, 21: 292-296, 2009.
Penna, B.; Thomé, S.; Martins, R.; Martins, G.; Lilenbaum, W. In vitro antimicrobial resistance of Pseudomonas aeruginosa isolated from canine otitis externa in Rio de Janeiro, Brazil. Brazilian Journal of Microbiology, 42: 1434-1436, 2011.
Petrie, A.; Watson, P. Estatística em ciência animal e veterinária. 2ª ed. São Paulo: Roca, 2009. 248 p.
Pye, C.C.; Yu, A.A.; Weese, J.S. Evaluation of biofilm production by Pseudomonas aeruginosa from canine ears and the impact of biofilm on antimicrobial susceptibility in vitro. Veterinary Dermatology, 24(4): 446-449, 2013.
Quinn, P.J.; Markey, B.K.; Leonard, F.C.; Hartigan, P.; Fanning, S.; FitzPatrick, E.S. Veterinary microbiology and microbial disease. 2nd ed. Oxford: Willey-Blackwell, 2011.
Rang, H.P.; Dale, M.M.; Ritter, J.M.; Flowe, R.J.; Henderson, G. Fármacos Antibacterianos. In: Rang, H.P.; Dale, M.M.; Ritter, J.M.; Flowe, R.J.; Henderson, G. Rang & Dale farmacologia. 7ª ed. São Paulo: Elsevier, 2010. p. 622-637.
Saridomichelakis, M.N.; Farmaki, R.; Leontides, L.S.; Koutinas, A.F. Aetiology of canine otitis externa: A retrospective study of 100 cases. Veterinary Dermatology. 18(5): 341-343, 2007.
Wooltorton, E. Ototoxic effects from gentamicin ear drops. Canadian Medical Association, 167(1): 56, 2002.
Zur, G. Otitis externa en el perro. Consulta de Difusión Veterinaria, 22(212): 35-42, 2014.
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