Anthelmintic activity of Annona crassiflora leaves against Haemonchus contortus: part 1: in vitro inhibition of the hatchability and larval development

Autores/as

  • Marco Antonio Martins Caldeira Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil
  • Franciellen Morais-Costa Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil
  • Kaike Magno Macêdo Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil
  • Valdo Soares Martins Júnior Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil
  • Dheison Emanuel Augusto Pereira Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil
  • Ana Cláudia Maia Soares Universidade Estadual do Sudoeste da Bahia, Itapetinga-BA, Brasil
  • Isabela Parolis Martins Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil.
  • Idael Matheus Goes Lopes Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil.
  • Fernão Castro Braga Universidade Federal de Minas Gerais Federal, Belo Horizonte-MG, Brasil
  • Neide Judith Faria Oliveira Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil.
  • Eduardo Robson Duarte Universidade Federal de Minas Gerais, Montes Claros-MG, Brasil.

DOI:

https://doi.org/10.26605/medvet-v13n2-3067

Palabras clave:

Cerrado, nematode, flavonoids, quantitative coproculture.

Resumen

We evaluated the activity of Annona crassiflora leaves against Haemonchus contortus resistant to albendazol.  Aqueous (AE), ethanolic (EE) and ethyl acetate (EAE) extracts were produced and the predominant presence of flavonoids was observed in HPLC-DAD chromatograms. Initially we evaluated the larval development inhibition (LDI) of dry A. crassiflora leaf powder or its AE directly in fecal quantitative cultures. The efficacies of the extracts, with or without tannins, on egg hatching inhibition (EHI) were investigated reveling that the EE was the most effective (LC90 = 8.96 mg/mL). However, after tannin removal, AE showed the highest activity (LC90 = 4.27 mg/mL). In the LDI test, the LC90 of AE was < 6.25 mg/g of fecal culture and to leaf powder of leaves was 69.14 mg/g.  High efficacies of AE and EE for EHI were detected and the tannins were not the main active metabolites. The anthelmintic potential of this plant could be attributed to association between flavonoids and other metabolites.

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Publicado

2020-04-08

Cómo citar

Caldeira, M. A. M., Morais-Costa, F., Macêdo, K. M., Martins Júnior, V. S., Pereira, D. E. A., Soares, A. C. M., Martins, I. P., Lopes, I. M. G., Braga, F. C., Oliveira, N. J. F., & Duarte, E. R. (2020). Anthelmintic activity of Annona crassiflora leaves against Haemonchus contortus: part 1: in vitro inhibition of the hatchability and larval development. Medicina Veterinária, 13(2), 184–191. https://doi.org/10.26605/medvet-v13n2-3067

Número

Sección

Medicina Veterinária Preventiva