Use of grayscale histogram to assess testicular changes in ram caused by heat stress

Autores/as

DOI:

https://doi.org/10.26605/medvet-v17n2-5225

Palabras clave:

ovine; reproduction; semen; testicle

Resumen

Because it detects discrete changes in tissue density, ultrasound allows an early diagnosis of physiologic changes, especially when dealing with subclinical changes. aimed to evaluate the use of quantitative analysis of the gray scale of ultrasound images of ovine testicles as a method for early diagnosis of testicular changes caused by heat stress. Testicles from ten ovine were insulated and then evaluated regarding echogenicity, echotexture, anatomical measurements, and seminal characteristics. Echogenicity was the first variable to show changes during the insulation period, as well as the first one to show evidence of regeneration of those changes. There was a correlation (p < 0.05) between echogenicity and sperm vigor, motility, and mass sperm motility, as well as a correlation (p < 0.05) between echotexture and motility. Echogenicity, motility, vigor, and mass sperm motility decreased (p < 0.05) within four days of testicular insulation, and there was no change (p > 0.05) in echotexture. Testicular measurements were only altered (p < 0.05) after insulation; however, the structural integrity of the seminiferous tubules was not altered. Echogenicity is an efficient ultrasound parameter for the early diagnosis of a testicular degenerative process as well as for the early diagnosis of its regeneration.

Descargas

Los datos de descargas todavía no están disponibles.

Citas

Ayala, H.D. et al. Association of testicular echogenicity, scrotal circumference, testicular volume and testosterone concentration in buffaloes. Revista Brasileira de Medicina Veterinária, 38(4): 334-340, 2016.

Barros, D.V. et al. Evaluation of thermal comfort, physiological, hematological, and seminal features of buffalo bulls in an artificial insemination station in a tropical environment. Tropical Animal Health and Production, 47(5): 805-813, 2015.

Bastos, M.G.; Novaes, A.K.B.; Pazeli Jr., J.M.P. Traditional and ultrasound physical examinations: a hybrid approach to improve clinical care. Revista da Associação Médica Brasileira, 64: 474-480, 2018.

Belay, R.E.; Huang, G.O.; Shen, J.K.C.; Ko, E.Y.K. Diagnosis of clinical and subclinical varicocele: how has it evolved? Asian Journal of Andrology, 18(2): 182-185, 2016.

Camela, E.S. et al. Changes in testicular size, echotexture, and arterial blood flow associated with the attainment of puberty in Dorper rams raised in a subtropical climate. Reproduction in Domestic Animals, 54(2): 131-137, 2019.

Cardoso, F.M.; Queiroz, G.F. Duration of the cycle of the seminiferous epithelium and daily sperm production of Brazilian hairy rams. Animal Reproduction Science, 17(1-2): 77-84, 1988.

Chandolia, R.K. et al. Ultrasonography of the developing reproductive tract in ram lambs: effects of a GnRH agonist. Theriogenology, 48(1): 99-117, 1997.

Cóser, A.M.L.; Godinho, H.P.; Fonseca, V.O. Efeito de altas temperaturas sobre a espermatogênese de carneiros brancos deslanados em condições experimentais. Arquivos da Escola Superior de Veterinária. UFMG, 31(2): 147-154, 1979.

Durairajanayagam, D.; Agarwal, A.; Ong, C. Causes, effects and molecular mechanisms of testicular heat stress. Reproductive Biomedicine Online, 30(1): 14-27, 2015.

Florentino, C. et al. Efeito do tempo de insulação escrotal sobre os constituintes do plasma seminal de caprinos (Capra hircus L.) sem raça definida. Revista Ciência Veterinária nos Trópicos, 6(1): 39-45, 2003.

Fontoura, A.B.P. et al. Associations between feed efficiency, sexual maturity and fertility-related measures in young beef bulls. Animal, 10(1): 96-105, 2016.

Garcia, A.R. Influência de fatores ambientais sobre as características reprodutivas de búfalos do rio (Bubalus bubalis). Revista de Ciências Agrárias, 45: 1-13, 2006.

Garcia-Oliveros, L.N. et al. Heat stress effects on bovine sperm cells: a chronological approach to early findings. International Journal of Biometeorology, 64(8): 1367-1378, 2020.

Giffin, J.L.; Bartlewski, P.M.; Hahnel, A.C. Correlations among ultrasonographic and microscopic characteristics of prepubescent ram lamb testes. Experimental Biology and Medicine, 239(12): 1606-1618, 2014.

Hamilton, T.R.D.S. et al. Effect of heat stress on sperm DNA: protamine assessment in ram spermatozoa and testicle. Oxidative Medicine and Cellular Longevity, 2018: 1-14, 2018.

Henry, M.; Neves, J.P.; Jobim, M.I.M. Manual para exame andrológico e avaliação do sêmen animal. 3rd ed. Belo Horizonte: Colégio Brasileiro de Reprodução Animal, 2013. 104p.

Kahwage, P.R. et al. Assessment of body and scrotal thermoregulation and semen quality of hair sheep rams throughout the year in a tropical environment. Small Ruminant Research, 160: 72-80, 2018.

Lotti, F.; Maggi, M. Ultrasound of the male genital tract in relation to male reproductive health. Human Reproduction Update, 21(1): 56-83, 2015.

Mieusset, R. et al. Effects of heating the testes and epididymides of rams by scrotal insulation on fertility and embryonic mortality in ewes inseminated with frozen semen. Reproduction, 94(2): 337-343, 1992.

Moreira, E.P.; Moura, A.D.A.A.; Araújo, A.A.D. Efeitos da insulação escrotal sobre a biometria testicular e parâmetros seminais em carneiros da raça Santa Inês criados no Estado do Ceará. Revista Brasileira de Zootecnia, 30: 1704-1711, 2001.

Moule, G.R.; Waites, G.M.H. Seminal degeneration in the ram and its relation to the temperature of the scrotum. Reproduction, 5(3): 433-446, 1963.

Pierson, R.A.; Adams, G.P. Computer-assisted image analysis, diagnostic ultrasonography and ovulation induction: strange bedfellows. Theriogenology, 43(1): 105-112, 1995.

Rao, A.R.; Bane, A.; Gustafsson, B.K. Changes in the morphology of spermatozoa during their passage through the genital tract in dairy bulls with normal and impaired spermatogenesis. Theriogenology, 14(1): 1-12, 1980.

Rocha, D.R. et al. Effect of increased testicular temperature on seminal plasma proteome of the ram. Theriogenology, 84(8): 1291-1305, 2015.

Rojas, J.; Lizbeth, F.C.; Socorro, R.M. Stress and cell death in testicular cells. Andrology (Los Angel), 6(1): 183-90, 2017.

Santos, D.O.; Simplício, A.A. A insulação escrotal na fertilidade de caprinos adultos. Ciência Animal, 3(1): 14-25, 1993.

Santos, D.O.; Simplício, A.A.; Machado, R. Características escroto-testiculares e do ejaculado em bodes mestiços submetidos à insulaçäo escrotal. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 50(3): 287-91, 1998.

Setchell, B.P. The parkes lecture heat and the testis. Reproduction, 114(2): 179-194, 1998.

Sutovsky, P. et al. A putative, ubiquitin-dependent mechanism for the recognition and elimination of defective spermatozoa in the mammalian epididymis. Journal of Cell Science, 114(9): 1665-1675, 2001.

Publicado

2023-08-03

Cómo citar

Veloso Neto, H. F. ., Chaves, M. S., Ferreira-Silva, J. C. ., Vieira, J. I. T., Araujo, R. J. de O., Santos Filho, J. P. dos, Silva Filho, M. L. da, Freitas, V. J. de F., & Oliveira, M. A. L. (2023). Use of grayscale histogram to assess testicular changes in ram caused by heat stress . Medicina Veterinária, 17(2), 125–133. https://doi.org/10.26605/medvet-v17n2-5225

Número

Sección

Reprodução Animal