Assessment of the uterus in bovines with endometritis using Doppler ultrasound
DOI:
https://doi.org/10.26605/medvet-v19n3-7387Palabras clave:
Bovine medicine , Clinical reproduction , InfertilityResumen
Postpartum uterine diseases, such as metritis and endometritis, are highly prevalent in dairy cows, exerting a significant detrimental impact on reproductive performance and, consequently, economic activity. Cows with puerperal endometritis may show alterations in the hemodynamics of the uterine tissue and arteries. In this study, we applied Doppler ultrasound to describe the hemodynamic changes in the uterus of cows diagnosed with endometritis between 25 and 35 days postpartum. A total of 89 cows were divided into two experimental groups, infected (n=56) or uninfected (n=33), based on endometrial cytology. Clinical examinations, vaginoscopy, and Doppler ultrasound were performed. Cows with endometritis exhibited a larger cervix (P=0.040) and a larger left uterine horn (P=0.020) compared to healthy cows. Additionally, 78.6% of the cows with endometritis exhibited abnormal uterine discharge, whereas only 57.6% of healthy cows had the same condition (P=0.0005). Spectral Doppler evaluation of the uterine arteries revealed no differences between groups. However, a higher score or increase in uterine vascularization on endometrial Doppler was associated with Trueperella pyogenes (P=0.0003) and the presence of intrauterine heterogeneous content (P=0.0047). Furthermore, mesometrial Doppler findings correlated with endometrial Doppler (P<0.001), uterine bacterial presence (P=0.001), and intrauterine heterogeneous content (P=0.049). Doppler ultrasound offers a rapid evaluation method for uterine alterations. Moreover, it is a less invasive alternative to uterine biopsy and endometrial cytology and provides insights into fertility and uterine health.Descargas
Citas
Barlund, C.S. et al. Comparison of diagnostic techniques for postpartum endometritis in dairy cattle. Theriogenology, 69(6):714-723, 2008.
Bicalho, M.L. et al. Association between virulence factors of Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes and uterine diseases of dairy cows. Veterinary microbiology, 157(1-2): 125-131, 2011.
Boer, M. et al. Associations between intrauterine bacterial infection, reproductive tract inflammation, and reproductive performance in pasture-based dairy cows. Theriogenology, 83:1514-1515, 2015.
Bollwein, H.; Baumgartner, U.; Stolla, R. Transrectal Doppler sonography of uterine blood flow in cows during pregnancy. Theriogenology, 57(8):2053-2061, 2002.
Bollwein, H.; Lüttgenau, J.; Herzog, K. Bovine luteal blood flow: Basic mechanism and clinical relevance. Reproduction Fertility Development, 25(1):71-79, 2013.
Bollwein, H. et al. Transrectal color Doppler sonography of the uterine artery in cyclic mares. Theriogenology, 49:1483-1488, 2000.
Bollwein, H.; Heppelmann, M.; Lüttgenau, J. Ultrasonographic Doppler use for female reproduction management. Veterinary Clinics North American Small Animal Practice, 32(1):149-164, 2016.
Carvalho, C.F.; Cjammas, M.C.; Cerri, G.G. Princípios físicos do Doppler em ultra-sonografia. Ciência Rural, 38(3):872-879, 2008.
Couto Serrenho, R. et al. Controlled trial of the effect of negative dietary cation-anion difference on postpartum health of dairy cows. Journal of Dairy Science, 104(6):6929-6943, 2021.
Descoteaux L, Gnemmi G, Colloton J. Practical atlas of ruminant and camelid reproductive ultrasonography. 1st ed. Ames: Blackwell Publishing; 2009. p. 61-70
Dubuc, J. et al. Definitions and diagnosis of postpartum endometritis in dairy cows. Journal Dairy Science, 93(11):5225-5233, 2010.
Dubuc, J. et al. Effects of postpartum uterine diseases on milk production and culling in dairy cows. Journal Dairy Science, 94(3):1339-1346, 2011.
Gilbert, R.O. et al. Prevalence of endometritis and its effects on reproductive performance of dairy cows. Theriogenology, 64(9):1879-1888, 2005.
Ginther, O.J. Ultrasonic Imaging and Animal Reproduction: Color-Doppler Ultrasound. Cross Plains, WI: Equiservices Publishing, v.4, 258p, 2007.
Gröhn, Y.T.; Rajala-Schultz, P.J. Epidemiology of reproductive performance in dairy cows. Animal Reprodction Science, 60-61:605-614, 2000.
Grunert, E.; Birgel, E.H.; Vale, W.G. Patologia e clínica da reprodução dos animais mamíferos domésticos: Ginecologia. São Paulo: Varela, 551p, 2005.
Guido, F.C.L.; Guido, S.I.; Neto, J.E. Use of endometrial vascular perfusion by color Doppler, B-mode ultrasound and cytology for diagnosis of subclinical endometritis in Holstein cows. Revista Brasileira de Reprodução Animal, 44(4):159-167, 2020.
Heppelmann, M.; Krüger, L.; Leidl, H.B. Transrectal Doppler sonography of uterine blood flow during the first two weeks after parturition in Simmental heifers. Journal Veterinary Science, 14(3):323-327, 2013.
Honnens, A. et al. Uterine blood flow during the first 3 weeks of pregnancy in dairy cows. Theriogenology, 70:1048-1056, 2008.
Kasimanickam, R. et al. The effect of a single administration of cephapirin or cloprostenol on the reproductive performance of dairy cows with subclinical endometritis. Theriogenology, 63(3):818-830, 2005.
LeBlanc, S.J. et al. Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows. Journal Dairy Science, 85(9):2223-2236, 2002.
LeBlanc, S. J. Review: Postpartum reproductive disease and fertility in dairy cows. Animal, 17 Suppl 1:100781, 2023.
Madoz, L.V. et al. Endometrial cytology, biopsy and bacteriology for the diagnosis of subclinical endometritis in grazing dairy cows. Journal Dairy Science, 97(1):195-201, 2014.
Mateus, L. et al. Influence of puerperal uterine infection on uterine involution and postpartum ovarian activity in dairy cows. Reproduction domestic animal, 37(1):31-35, 2002.
Meira Jr., E.B.S. et al. Comparison of ultrasound and histopathology for diagnosis of endometritis in Holstein-Friesian cows. Journal Dairy Science, 95(2): 6969-6973, 2012.
Mello, R.C. Perdas reprodutivas em fêmeas bovinas. Agropecuária Científica no Semiárido, 10(4): 7-23, 2014.
Morrow, D.A. et al. Postpartum ovarian activity and uterine involution in dairy cattle. Journal American Veterinary Medical Association, 149:1596-1609, 1966.
Oral, H. et al. Comparison of the cytobrush technique, vaginoscopy and transrectal ultrasound methods for the diagnosis of postpartum endometritis in cows. Journal Animal Veterinary Advances, 8(7):1252-1255, 2009.
Piersanti, R.L. et al. A model of clinical endometritis in Holstein heifers using pathogenic Escherichia coli and Trueperella pyogenes. Journal of Dairy Science, 102(3):2686-2697, 2019.
Potter, T.J. et al. Risk factors for clinical endometritis in postpartum dairy cattle. Theriogenology, 74:127-134, 2010.
Radostits, O.M. et al. Veterinary Medicine: a textbook of diseases of cattle, sheep, pigs, goats and horses. 10th ed. Philadelphia: WB Saunders, 2007, p. 1455-1470.
Santos, T.M.; Gilbert, R.O.; Bicalho, R.C. Metagenomic analysis of the uterine bacterial microbiota in healthy and metritic postpartum dairy cows. Journal Dairy Science, 94(1):291-302, 2011.
Sheldon, I.M. et al. Defining postpartum uterine disease in cattle. Theriogenology, 65(8):1516-1530, 2006.
Slama, H.; Vaillancourt, D.; Goff, A.K. Pathophysiology of puerperal period: relationship between prostaglandin E2 (PGE2) and uterine involution in the cow. Theriogenology, 36(6):1071-1090, 1991.
Steer, C.V. et al. Midluteal-phase vaginal color Doppler assessment of uterine artery impedance in subfertile population. Fertil Steril, 61(1):53-58, 1994.
Descargas
Publicado
Cómo citar
Número
Sección
Licencia
Derechos de autor 2025 Bruno Leonardo Mendonça Ribeiro, Enoch Brandão de Souza Meira Junior, Mario Augusto Reyes Aleman, Lucas Alencar Fernandes Beserra, Alessandra Figueiredo de Castro Nassar, LILIAN GREGORY
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.
- A Revista de Medicina Veterinária permite que o autor retenha os direitos de publicação sem restrições, utilizando para tal a licença Creative Commons CC BY-NC-SA 4.0.
- De acordo com os termos seguintes:
- Atribuição — Você deve dar o crédito apropriado, prover um link para a licença e indicar se mudanças foram feitas. Você deve fazê-lo em qualquer circunstância razoável, mas de nenhuma maneira que sugira que o licenciante apoia você ou o seu uso.
- NãoComercial — Você não pode usar o material para fins comerciais.
- CompartilhaIgual — Se você remixar, transformar, ou criar a partir do material, tem de distribuir as suas contribuições sob a mesma licença que o original.
- Sem restrições adicionais — Você não pode aplicar termos jurídicos ou medidas de caráter tecnológico que restrinjam legalmente outros de fazerem algo que a licença permita.
