Effect of in ovo feeding of L-glutamine + ISP to chick embryos
DOI:
https://doi.org/10.26605/medvet-v13n4-3669Palavras-chave:
amino acid, biotechnology, embryo mortality, hatchability, in ovo feedingResumo
In ovo feeding (IOF) is the biotechnology that injects nutrients into the amnion of the avian embryo may cause an increase in hatchability and gastrointestinal development, and affecting the serum metabolism. This hypothesis was evaluated using 5 IOF solutions containing L-glutamine and L-glutamine + ISP (Isolated Soy Protein). 300 fertile eggs Rhode Island Red (breeders with 43-weeks) were distributed in a completely randomized experimental design with the treatments constituted by two controls (intact egg and IOF control with saline solution) and three solutions containing L-glutamine and L-glutamine + ISP, with 60 replicates (eggs) each. Data collected were subjected to the Tukey test at 5% of significance. There was a significantly lower (p<0.01) in hatchability from the IOF of L-glutamine + ISP, and a consequent increase in the intermediary embryo mortality. The yolk sac was higher (p<0.01) in chicks subjected to solutions containing 0.5% L-glutamine and 0.5% L-glutamine + 1.0% ISP, whereas all in ovo embryos presented great (p<0.05) development of heart and pancreas than intact eggs. In ovo fed embryos at 0.5% L-glutamine and 0.5% L-glutamine + 1.0% ISP presented great developed of gastrointestinal tract than intact eggs. In conclusion, the results of this study indicated that 0.5% L-glutamine may be used to IOF in chick embryos without a negative influence on chick weight and gastrointestinal tract development, acting as a metabolism regulator and obtaining better hatchability.Downloads
Referências
Buddington, R.K.; Diamond, J.M. Ontogenetic development of intestinal nutrient transporters. Annual Review of Physiology, 51: 601–619, 1989.
Damasceno, J.L.; Cruz, F.G.G.; Melo, R.D.; Feijo, J.C.; Rufino, J.P.F.; Valentim, F.M.; Oliveira, J.P.C. Inoculação de proteína isolada de soja em ovos embrionados oriundos de matrizes semipesadas com diferentes idades. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 69(5): 1259-1266, 2017.
Diamond, J.M.; Karasov, W.H. Adaptive regulation of intestinal nutrient transporters. Proceedings of the National Academy of Sciences USA, 84: 2242–2245, 1987.
Ferraris, R.P.; Diamond, J.M. Specific regulation of intestinal nutrient transporters by their dietary substrates. Annual Review of Physiology, 51: 125–141, 1989.
Ferraris, R.P.; Villenas, S.A.; Hirayama, B.A.; Diamond, J.M. Effect of diet on glucose transporter site density along the intestinal crypt-villus axis. American Journal of Physiology, 262: 1060-1068, 1992.
Foye, O.T.; Uni, Z.; Ferket, P.R. Effect of In Ovo Feeding Egg White Protein, β-Hydroxy-β-Methylbutyrate, and Carbohydrates on Glycogen Status and Neonatal Growth of Turkeys. Poultry Science, 85(7): 1185-1192, 2006.
Foye, O.T.; Uni, Z.; Ferket, P.R. The Effects of In Ovo Feeding Arginine, β-Hydroxy-β-Methyl-Butyrate, and Protein on Jejunal Digestive and Absorptive Activity in Embryonic and Neonatal Turkey Poults. Poultry Science, 86(12): 2343–2349, 2007.
Geyra, A.; Uni, Z.; Sklan, D. Enterocyte dynamics and mucosal development in the posthatch chick. Poultry Science, 80(6): 776-782, 2001.
Gonzales, E.; Cruz, C.P.; Leandro, N.S.M.; Strighini, J.H.; Brito, A.B. In Ovo Supplementation of 25(OH)D3 to Broiler Embryos. Brazilian Journal of Poultry Science, 15(3): 169-286, 2013.
Harvey, S.; Johnson, C.D.M.; Sanders, E.J. Growth hormone in neural tissue of the chick embryo. Journal of Endocrinology, 169(3): 487-496, 2001.
Ipek, A.; Sahan, U.; Yilmaz, B. The effects of in ovo ascorbic acid and glucose injection in broiler breeder eggs on hatchability and chick weight. Archives Geflugelk, 68: 132-135, 2004.
Jepson, M.M.; Bates, P.C.; Broadbent, P.; Pell, J.M.; Millward, D.J. Relationship between glutamine concentration and protein synthesis in rat skeletal muscle. American Journal of Physiology, 255(2): 166-172, 1988.
Jochemsen, P.; Jeurissen, S.H.M. The location and uptake of in ovo injected soluble and particulate substances in the chicken. Poultry Science, 81: 1811-1817, 2002.
Karasov, W.H.; Solberg, D.H.; Diamond, J.M. Dependence of intestinal amino acid uptake on dietary protein or amino acid levels. American Journal of Physiology, 5: 252: 614-625, 1987.
Leitão, R.A.; Leandro, N.S.M.; Stringhini, J.H.; Café, M.B.; Andrade, M.A. Inoculação de maltose, sacarose ou glicose em ovos embrionados de baixo peso. Acta Scientiarum. Animal Sciences, 32(1): 93-100, 2010.
Leitão, R.A.; Leandro, N.S.M.; Stringhini, J.H.; Café, M.B.; Matos, M.S.; Andrade, M.A. Inoculação de maltose e/ou sacarose em ovos leves embrionados. Ciência Animal Brasileira, 15(1): 55-63, 2014.
Maiorka, A.; Silva, A.V.F.; Santin, E.; Borges, S.A.; Boleli, I.C.; Macari, M. Influência da suplementação de glutamina sobre o desempenho e o desenvolvimento de vilos e criptas do intestino delgado de frangos. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 52(5): 487-490, 2000.
Nelson, D.L.; Cox, M.M. Princípios de Bioquímica de Lehninger. 6ª Ed. Porto Alegre: Artmed, 2014. 1328p.
Ohta, Y.; Kidd, M.T. Optimum site for in ovo amino acids injection in broiler breeders’ eggs. Poultry Science, 80: 1425-1429, 2001.
Pedroso, A.A.; Chaves, L.S.; Lopes, K.L.A.M.; Leandro, N.S.M.; Café, M.B.; Stringhini, J.H. Inoculação de nutrientes em ovos de matrizes pesadas. Brazilian Journal of Poultry Science, 35(5): 2018-2026, 2006.
Ray, E.C.; Avissar, N.E.; Vukcevic, D.; Toia, L.; Ryan, C.K.; Berlanga-Acosta, J.; Sax, H.C. Growth hormone and epidermal growth factor together enhance amino acid transport systems B and A is Remnant small intestine after massive enterectomy. Journal of Surgical Research, 115(1): 164-170, 2003.
Silva, A.V.F.; Borges, S.A.; Maiorka, A.; Giviziez, P.E.N.; Rocha, C.; Macari, M. Ornitine Decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation. Brazilian Journal of Poultry Science, 9(2): 111-116, 2007.
Solberg, D.H.; Diamond, J.M. Comparison of different dietary sugars as inducers of intestinal sugar transporters. American Journal of Physiology, 252(4): 574-584, 1987.
Tako, E.; Ferket, P.R.; Uni, Z. Effects of in ovo feeding of carbohydrates and β-hydroxy-β-methylbutyrate on the development of chicken intestine. Poultry Science, 83(12): 2023-2028, 2004.
Torras-Llort, M.; Soriano-Garcia, J.F.; Ferrer, R.; Moreto, M. Effect of a lysine-enriched diet on L-lysine transport by the brush-border membrane of the chicken jejunum. American Journal of Physiology, 274(1): 69-75, 1998.
Uni, Z.; Ferket, P. Enhancement of development of oviparous species by in ovo feeding. US Regular Patent US 6,592,878 B2. North Carolina State Univ., Raleigh and Yissum Res. Dev. Co., Hebrew Univ. Jerusalem, Israel, 2003.
Uni, Z.; Ferket, P.R.; Tako, E.; Kedar, O. In ovo feeding improves energy status of late-term chicken embryos. Poultry Science, 84(5): 764-770, 2005.
Welborne, T.C. Increased plasma bicarbonate and growth hormone after an oral glutamine load. American Journal of Clinical Nutrition, 61(5): 1058-1061, 1995.
Downloads
Publicado
Como Citar
Edição
Seção
Licença
- A Revista de Medicina Veterinária permite que o autor retenha os direitos de publicação sem restrições, utilizando para tal a licença Creative Commons CC BY-NC-SA 4.0.
- De acordo com os termos seguintes:
- Atribuição — Você deve dar o crédito apropriado, prover um link para a licença e indicar se mudanças foram feitas. Você deve fazê-lo em qualquer circunstância razoável, mas de nenhuma maneira que sugira que o licenciante apoia você ou o seu uso.
- NãoComercial — Você não pode usar o material para fins comerciais.
- CompartilhaIgual — Se você remixar, transformar, ou criar a partir do material, tem de distribuir as suas contribuições sob a mesma licença que o original.
- Sem restrições adicionais — Você não pode aplicar termos jurídicos ou medidas de caráter tecnológico que restrinjam legalmente outros de fazerem algo que a licença permita.
